PCR、DNA荧光染色法与培养法在支原体检测中的比较  

Comparison of PCR, DNA Fluorescent and Cultivation Used for Detection of Mycoplasmas

作　　者：姜典才[1] 高光[1] 张宁[1] 尹红章[1] 李德富[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》1995年第4期179-181,共3页Chinese Journal of Biologicals

摘　　要：用PCR、DNA荧光染色法及培养法，对46株细胞培养物中污染的支原体进行检测，对实验过程及所获得的实验结果进行分析，从实验的可行性、敏感性、可靠性三方面对3种方法进行了评估分析。以便选择支原体检测方法提供参考。The mycoplasmas col1taminated in 46 strains of cell culture were detected byPCR, DNA fluorescent and cultivation. After experimental process and result were analysed,the 3 methods were evaluted in feasibility, sensitivity and reliability-

关 键 词：DNA荧光染色法 培养法 聚合酶链反应 支原体 

分 类 号：R392－33[医药卫生—免疫学]