应用牛腹腔唇乳突丝虫（Setaria labiatopapillosa）抗原的ELISA检测班氏丝虫病人  

A Study of the Sensitivity and Specificity of the ELISA with Bovine Setaria G Antigen in Determination of theSerum Antibody in the Human Bancroftian Filariosis

作　　者：徐礼周[1] 冯子玲[1] 叶萍[1] 钟志萍[1] 周勇志[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,杭州商学院

出　　处：《中国兽医学报》1995年第3期264-266,共3页Chinese Journal of Veterinary Science

摘　　要：应用牛腹腔唇乳突丝虫（Ｓｅｔａｒｉａｌａｂｉａｔｏｐａｐｉｌｌｏｓａ）提纯抗原（简称牛丝虫Ｇ抗原）和抗人ＩｇＧ酶标记物的酶联免疫吸附试验，检测２２７例班氏丝虫病人血清的阳性率为９５．５９％；检测１３２例健康人血清的假阳性率为２．２７％，１０例血吸虫病人血清的阳性率为０。应用牛丝虫Ｇ抗原和马来丝虫抗原同步检测５０例班氏丝虫病人血清的阳性率，前者为９８％，后者为８６％，二者有显著差异（ｐ＜０．０５）。因此认为，牛丝虫Ｇ抗原可以作为人丝虫病的异种抗原诊断试剂。The purified G antigen of bovine setaria and the enzyme-labelled antihu- man IgG were used in ELISA to determine the serum of the patients infested with Filaria bancrofti, abbreviated as GAg-ELISA in diagnosis of the human bancroftian fi- lariosis. First the positive rates of bovine setaria G antigen (GAg)with antigen of W uchereria malayi were synehronously compared as follows : the former was 98%and the later 86%when detections of 50 patients'serum of bancroftian filariosis were under- taken.Both statistical data showed that the bovine G antigen was better than Brugia malayi antigen( P <0.05).The second result showed that the former (GAg)positive rate of 227 patients with bancroftian filariosis was 95.59%,the false positive rate in 132 cases of healthy persons was 2.27%,the cross reaction rates in 10 patients with schisto- somiasis and 15 patients with systicercosis were all 0%. Thus, it suggested that the bovine setaria G antigen may be exploited as a diagnostic reagent of heterogenous anti- gen for human filariasis.

关 键 词：牛唇乳突丝虫 抗原 ELISA 班氏丝虫病 人血清 

分 类 号：S852.731[农业科学—基础兽医学] R446.61[农业科学—兽医学]