用抗新城疫病毒膜蛋白特异多肽抗体鉴别强毒株和弱毒株  

Antipeptide Antibodies against NDV Membrane Glycopro-tein in Analysis of Virulent and Attenuated Strains

作　　者：王树双[1] 孙书华[1] 吴时友[1] 

机构地区：[1]山东青岛农业部动物检疫所

出　　处：《中国兽医学报》1995年第3期239-242,共4页Chinese Journal of Veterinary Science

摘　　要：利用ＪｅｆｆＧｏｒｍａｎ建立的用抗新城疫病毒（ＮＤＶ）膜蛋白特异多肽抗体鉴别强弱毒株的方法，对国内的Ⅰ系、Ⅱ系、ＬａＳｏｔａ和两株分离毒进行了毒力分析。结果证明，上述毒株的毒力顺序为：Ⅰ系＞Ⅱ系和ＬａＳｏｔａ＞Ｖ４（澳大利亚弱毒疫苗株）＞临床分离株（青岛）。其结论为：Ⅰ系毒株与澳大利亚维多利亚分离株（ＡＶ）的Ｆ２蛋白片段有类似的氨基酸序列，属中发型毒株。虽然Ⅱ系和ＬａＳｏｔａ比Ｖ４和临床分离株（青岛）毒力稍强，但认为这４株均为缓发型毒株。该方法敏感、特异，结果精确可靠，不仅可用于临床分离野毒的致病性分析，而且也可用于疫苗株研究中毒力的评价。Five Strains of NDV(Ⅰ,Ⅱ, La Sota,NDC and G strains)were anal- ysed by the method of antipeptide antibodies against NDV membrane glycoprotein devel- oped by Gorman. The results showed their virulence in the following order,Ⅰ> Ⅱ and La Sota>V4>NDC, suggesting that Ⅰ strain had the similar AA sequence with the F2 fragment of Australian Victorian strain(AV)and is a mesogenic pathotype strain,Al- though the Ⅱ strain and La Sota are more virulent compared with the V4 and NDC,all the 4 strains are the lentogenic pathotype strains. The method is sensitive and specific and its results are exact and reliable. It can be applied both in the analysis of field iso- lates for their pathogenicity and in the evaluation of virulence of expectant vaccine Strains.

关 键 词：新城疫 病毒 膜蛋白 抗多肽抗体 毒株 家禽 

分 类 号：S852.65[农业科学—基础兽医学]