伊氏锥虫和布氏锥虫动基体DNA酶切电泳比较  

The Electrophoric Comparison of Restriction Endonuclease Analyzed KDNA of Trypanosoma brucei and Trypanosoma evansi

作　　者：郑韧坚[1] 王云飞[1] 沈杰[1] 周勇志[1] 钟淑梅[1] 

机构地区：[1]中国农科院上海家畜寄生虫病研究所

出　　处：《中国兽医杂志》1995年第7期9-10,共2页Chinese Journal of Veterinary Medicine

摘　　要：限制性内切酶ＭｂｏＩ、ＤｄｅＩ、ＨｉｎｆＩ和ＴａｑＩ对市氏锥虫ＫＤＮＡ进行酶切后，电泳中均显示出多条ＤＮＡ区带，其总Ｋｂ数约等于２０ｋｂ，内各限制酶对伊氏锥虫ＫＤＮＡ消化后均显示出１至２条区带，总和约１ｋｂ，明显区别于布氏锥虫。The KDNA )f T,brucei and T. evansi was analyzed by restrictionendonuclease MboI. DdeI.HinfI.TaqI.After electrophoresis,the sample of T.brucei showed many DNA bands and the total base pair was about 20kb,and thesample of T,evansi showed only 1 to 2 bands in electrophoric gel and the totalbase pair was about lkb.It was evidently different from T. brucei.

关 键 词：伊氏锥虫 布氏锥虫 限制性内切酶 动基体 

分 类 号：S852.72[农业科学—基础兽医学]