两株抗M和两株抗N单克隆抗体的血型血清学分析  

Mood Group Serological Identification of Monocolonal Antibodies Anti-M and Anti-N

作　　者：李世忠 李岩 李勇 袁琳 于晓丽 张雪松 陈晶[2] 

机构地区：[1]长春市输血研究所,130051 [2]吉林市中心血站

出　　处：《中国输血杂志》1995年第3期124-126,共3页Chinese Journal of Blood Transfusion

摘　　要：用人O型红细胞免疫Balb/C小鼠,取其脾细胞与鼠骨髓瘤细胞系Ns-1以PEG为助融剂进行融合。用人的标准谱细胞筛选,一次融合得到2株抗M杂交瘤细胞株(2A1和3F6)及2株抗N杂交瘤细胞株(4E3和7G5),均能稳定地分泌IgM型抗体,与人血清试剂平行鉴定300份血样,测定结果完全一致。4E3单抗完全不被O型M红细胞所吸收,7G5能被吸收。The BALB/c mice were immunized with human O blood cells. Their spleen cells were fused with murine myeloma cells Ns-1 with PEG. The monoclonal antibodies were identified with human panelled cells. Two clones secreting monoclonal anti-M(2Al and 3F6)and another two clones secreting monoclonal anti-N(4E3 and 7G5)were identified. All these clones could consistently secrete IgM antibodies. In typing 300 different RBC samples,the specificity of antibodies were identical to those of commercial humen anti-M and anti-N typing sere reagent. Significantly, anti-N reagent 4E3 differed from anti-N reagent 7G5, which the former one couldn't be absorbed by M erythrocytes at all,while the latter one absorbed by M erythroeytes.

关 键 词：抗-M 抗-N 单克隆抗体 血型血清学 

分 类 号：R392.11[医药卫生—免疫学]