ApoA_Ⅰ基因多态性与NIDDM关系的研究  

The relationship between ApoA_Ⅰgene ploymorphism and NIDDM

作　　者：马向华[1] 陈家伟[1] 柴建华[1] 叶岷玮 沈捷[1] 顾杨洪[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,上海复旦大学遗传研究所

出　　处：《中国糖尿病杂志》1995年第2期69-72,共4页Chinese Journal of Diabetes

摘　　要：采用限制性片段长度多态性（ＲＦＬＰ）技术，对９０例中国汉族人进行分析，发现载脂蛋白ＡⅠ（ＡｐｏＡⅠ）Ｍ１等位基因频率在体重指数（ＢＭＩ）≥２４的非胰岛素依赖型糖尿病（ＮＩＤＤＭ）与正常对照组之间、在ＢＭＩ≥２４的ＮＩＤＤＭ与ＢＭＩ＜２４的ＮＩＤＤＭ之间、在动脉硬化（ＡＳ）与正常对照组之间均存在显著性差异。此外，ＮＩＤＤＭ伴ＡＳ（ＮＡ）的等位基因频率分布与ＮＩＤＤＭ相似，与ＡＳ不同。根据上述结果，认为：ＡｐｏＡⅠ基因与ＢＭＩ≥２４的ＮＩＤＤＭ相关联；ＢＭＩ≥２４的ＮＩＤＤＭ与ＢＭＩ＜２４的ＮＩＤＤＭ之间存在着遗传异质性；ＡｐｏＡⅠ基因与ＡＳ相关联；在ＡｐｏＡⅠ基因水平上，未见ＮＩＤＤＭ与ＡＳ相关联。The restriction fragment length polymorphism (RFLP) technique was used to study 90 Chinese of Han nationality. The subjects were divided into four groups: Non-insulin-dependent Diabetes mellitus (NIDDM),Atherosclerosis (AS),NIDDM with AS (NA) and control subjects (Contronl).Each group was subdivided into BMI≥24 and BMI<24 based on body mass index (BMI). Genomic DNA was digested with the restriction endonuclease Mspl and hybridized with 32p-labelled ApoAⅠ cDNA probe. The results showed that there were significant differences between NIDDM with BMI≥24and Control with BMI≥24,NIDDM with BMI≥24 and NIDDM with BMI<24 and also between AS and control.The allele frequency of NIDDM with AS NA was similar to that of NIDDM but different from that of AS. We believe that there were correlations of ApoAⅠ gene with NIDDM with BMI≥24 as well as AS. There were genetic heterogeneity between NIDDM with BMI≥24 andNIDDM with BMI<24. There were, however, no relationship between NIDDM and AS in ApoAⅠ gene.

关 键 词：NIDDM 载脂蛋白AI 糖尿病 基因多态性 

分 类 号：R587.102[医药卫生—内分泌]