家兔休克再灌注胃粘膜氧自由基的直接测定与其意义  

Direct measurement of oxygen free radicals in gastric perioc We mucosa induced by shock-reperfusion

作　　者：徐泽宽[1] 张保康[1] 

机构地区：[1]南京医科大学一附院外科

出　　处：《中国危重病急救医学》1995年第1期1-4,共4页Chinese Critical Care Medicine

摘　　要：采用自旋捕捉剂（ＰＢＮ）以稳定高度活性的自由基，并用电子自旋共振（ＥｌｅｃｔｒｏｎＳｐｉｎＲｅｓｏ－ｎａｎｃｅ，ＥＳＲ）技术直接测定休克再灌注胃粘膜不同时限氧自由基（ＯｘｙｇｅｎＦｒｅｅＲｅｄｉｃａｌｓ，ＯＦＲ）的含量变化，并用肉眼和光镜观察胃粘膜形态变化。实验家兔２６只随机分为４组。结果显示：休克９０ｍｉｎ组的胃粘膜ＯＦＲ含量已显著升高，假休克组相比具有统计学意义（Ｐ＜０．０５），再灌注后胃粘膜ＯＦＲ含量进一步增高，并随时间延长而进一步递升，与假休克组相比均具有非常显著意义（Ｐ＜０．０１）。病理改变严重程度与ＯＦＲ含量变化趋势基本一致。Electron spin resonance(ESR )spectroscopy andspin trapping agent phenyl-n-tert-butylnitrone(PBN)were applied to capture and detect variationsof oxygen free rad icals(OFR)in gastric m ucosaperi-shock-reperfusion injury. The pathology of gas-tric mucosa was simultaneously observed,26 rabbitswere divided into four groups. The results ind icatedthat,comparing with sham-shock group,concentra-tions of OFR were significantly increased(P<0.05)90 min after shock,and further augmented afterreperfusion(P<0.01).The severity of pathologicchances was in proportion to the concentration ofOFR.

关 键 词：休克 再灌注 自由基 氧 电子自旋共振 

分 类 号：R441.9[医药卫生—诊断学] R605.971[医药卫生—临床医学]