应用微菌落技术对细菌进行快速定量测定  

Rapid Enumeration of Bacteria by Microcolony Technique

作　　者：许欣[1] 裴晓方[1] 叶梅君[1] 康莉[2] 沈一民[3] 林怡伶[1] 

机构地区：[1]华西医科大学公共卫生学院,四川成都610041 [2]华西医科大学公共卫生学院卫检专业 [3]成都军区总医院检验科

出　　处：《中国卫生检验杂志》1995年第1期9-11,共3页Chinese Journal of Health Laboratory Technology

摘　　要：本文利用微菌落技术建立了一种对细菌进行快速定量测定的方法。其基本方法为将待测标本定量接种于醋酸纤维素薄膜上,经37℃5h培养,染色后对标本区的薇菌落作显微镜计数。用本法与平板计数法同时对大肠杆菌和金黄色葡萄球菌悬液进行测定,得到很好的相关性;对72份尿液标本测定结果作统计学分析,二者差别无显著性。This paper described a microcolony method for rapid enumeration of bacteria. In brief, a certain amount of samples were inoculated on the cellulose ? **, acetate membrane which had been placed on the incubation pad- Aftt-r incubated for 5h at 37 C. the membrane was dried and stained. The number of micro- colonies were counted under the microscope- The microcolony count of bacteria on the membrane correlated well with the plate count of E. coli, S. aureus and bacteria in urine.

关 键 词：微菌落 细菌 定量测定 卫生检验 

分 类 号：R115[医药卫生—公共卫生与预防医学]