铜与维生素C协同灭活大肠杆菌噬菌体的机理  

MECHANISM OF SYNERGETIC INACTIVATION OF COLIPHAGE f_2 BY COPPER AND VITAMINC

作　　者：梁增辉[1] 杨宗芬[1] 王福玉[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所

出　　处：《中国消毒学杂志》1995年第1期1-5,共5页Chinese Journal of Disinfection

基　　金：国家自然科学基金(38970630)

摘　　要：用电子自旋共振仪检测维生素Ｃ和硫酸铜混合液的自由基波谱，及其灭活ｆ＿２噬菌体作用。结果表明，维生素Ｃ自由基有较强的灭活噬菌体作用。硫酸铜可提高维生素Ｃ自由基强度，并加速其灭活噬菌体的作用。Vitamin C has certain inactivating effect on viruses,which is considered as the result offormation of free radicals in solution. In order to understand the mechanism of synergism ofcopper with vitamin C in inactivating coliphage f_2, the spectrum of free radicals in mixed so-lution of vitamin C and CuSO_4 was examined and the relation between the free radicals andinactivation of coliphage f_2 was observed. The results indicated that no signal of OH·radical and O'_2 radical was detected in themixed solution of vitamin C (10￣(-3)～10￣(-2)mol / L) and copper sulfate (10￣ (-5)～10￣(-2)mol/L),while the spectrum of double-peak-shaped free radicals of vitamin C was detected.Thestrength of vitamin C free radical was higher than that in the solution of vitamin C alone. Thetest for inactivation of phage f_2 indicated that the free radicals of vitamin C had stronginactivating effect on the phage,The higher the strength of vitamin C free radicals,the betterthe inactivating efficacy.

关 键 词：维生素C 铜 协同作用 噬菌体 大肠杆菌 药理学 

分 类 号：R977.23[医药卫生—药品] R977.5[医药卫生—药学]