在乙型肝炎表面抗原破坏试验中对ELISA法检验结果影响因素的研究  

STUDY ON THE FACTORS INFLUENCING RESULTS OF EXAMINATION WITH ELISA IN TEST FOR DESTRUCTION OF HBsAGg

作　　者：李新武[1] 袁洽[1] 孙守宏[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所

出　　处：《中国消毒学杂志》1995年第2期89-93,共5页Chinese Journal of Disinfection

摘　　要：试验表明，在ＨＢｓＡｇ破坏试验中，用ＥＬＩＳＡ法检测时，样品含小牛血清量愈多检出ＨＢｓＡｇ的灵敏度愈高。某些阴离子表面活性剂使检测结果呈假阴性，加适宜中和剂可将之克服。新洁尔灭与脂肪醇聚氧乙烯醚可使检测结果呈假阳性。所试中和剂中，仅亚硫酸钠与半胱氨酸可使测得的Ｓ／Ｎ值下降，其余无明显影响。In order to know the influences of calf serum. surfactants and common neutralizers onthe resultS of examining HBsAg disinfection efficacy with ELISA,experimental observationwas carried out.The results showed that the higher the calf serum content of the sample,themore sensitive the test to detect HBsAg.The test for influences of surfactants and neutralizersindicated that:① presence of some anion surfactants influenced greatly the result of exami-nation,even caused false negative result,but use of suitable neutralizer could remove the in-fluence after neutralizing the effect of surfactant;②presence of bromogeramine(cationsurfactant)and aliphatic alcohol polyoxyethylene ether(nonionic surfactant)could causefalse positive result of the examination;③of the neutralizers tested,lecithin,tween 80,sodiumthiosulfate and glycine influenced the result of ELISA examination insignificantly,while so-dium sulfite and cysteine had great influence and caused obvious decrease in S/N valuemeasured,thus resulting in occurrence of false negative result.

关 键 词：乙型肝炎 表面抗原 ELISA 破坏试验 消毒剂 

分 类 号：R187.1[医药卫生—流行病学] R373.2[医药卫生—公共卫生与预防医学]