几丁质室内植入雪旺氏细胞对神经再生影响实验研究  

ABSTKACTEXPERIMENTAL STUDY ON THE GROWTH OF NERVE AXON ENHANCED BY CHITINREGENERATION CHAMBER WITH CRUDE SCHWANN CELLS

作　　者：苟三怀[1] 侯春林[1] 臧鸿声[1] 李晓华[1] 

机构地区：[1]上海第二军医大学长征医院骨科

出　　处：《中国修复重建外科杂志》1995年第2期108-111,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金

摘　　要：采用生物材料几丁质制成的管道作为神经再生室，对大鼠坐骨神经缺损１２ｍｍ进行桥接。在几丁质室内植入粗品雪旺氏细胞（ｒｓｃ）和不植入ｒｓｃ，并与骨能肌桥接进行了比较。术后４、８、１２周进行大体观察、显微解剖、神经电生理测试、辣根过氧化酶（ＨＲＰ）逆行示踪、组织学检查和电镜观察。结果：植入和不植入ｒｓｃ在术后８周近端再生神经纤维均与远端纤维连接，但不植入ｒｓｃ再生神经的形态、方式和大鼠肢体功能的恢复明显优于植入ｒｓｃ和肌桥。he biomaterial. chitin, was used to create a nerve regeneration chamber for bridging heal-ing experiment of sciatic nerve of rats having a defect of 12mm. The crude Schwann cells wereintroduced into the chambers in one group and the other group had no crude Schwann cells in the chanmber and the results of the two groups were compared with those having the nerve defectsbridged with skeletal muscles. The specimens were observed by macroscopic,micro-dissection.electrophysiologic testing,HRP retro-grade labelling, histofogic and eiectron microscopic exami-nations at 4.8.and 12 weeks after the operation。 The results showed that at the 8th week,theregenerating nerve fibers from the ceptialad ends had united with the fibers of the caudal ends ofthe divided nerves either the crude Schwann eclls were introduced or not, but the morphology ofthe regenerating nerve.the way of regeneration and the recovery of the function of the extremi-ties were far superior in the group that no cruds Schwann cells had been introduced than thosewith crude Schwann cell introduced and those bridged by skeletal muscles.

关 键 词：几丁质 周围神经缺损 雪旺氏细胞 神经再生 

分 类 号：R651.3[医药卫生—外科学]