应用间接免疫荧光法检测鸡传染性贫血病病毒抗体的研究  

Studies on the Detection of Antibodies Against Chicken Infectious Anemia Virus by an Indirect Immunofluorescent Antibody Technique

作　　者：崔现兰 周方红[1] 幸桂香[1] 李锋[1] 曲立新[1] 李成[1] 

机构地区：[1]中国农科院哈尔滨兽医研究所

出　　处：《中国畜禽传染病》1995年第5期4-6,共3页

摘　　要：用鸡传染性贫血病病毒(CIAV)MSBI-TK5803毒株感染的MDCC-MSB_1细胞涂片为抗原,建立了检测CIAV抗体的间接免疫荧光法(HlF)。应用该HFA方法对人工感染的SPF鸡进行了检测15只1日龄SPF鸡在接种后第7、14天时均呈阴性反应,21天时2只鸡呈弱阳性反应,28天时均呈弱阳性反应,35天时呈明显阳性反应;14只40日龄SPF鸡在感染CIAV后第4天均呈阳性反应,6个月时检测仍呈阳性反应。对我国大连、沈阳和哈尔滨三地区部分鸡场鸡群进性CIAV抗体检测,结果表明我国鸡群中存在CIAV抗体。An indirect immunofluorescent antibody technique (UFA) for the detection of antibodies against chicken infectious anemia virus (CIAV) was developed using CIAV-infected cell smears. 15 sera of 1 day-old SPF chicks inoculated with CIAV gave negative results 7 and 14 days postinoculation (PI). 2 and 15 sera of the 15 chicks gave weak positive results 21 and 28 days PI respectively. All the 15 sera of 15 chicks were detected positively 35 days PI. All the 14 sera from 14 40-day-old SPF chickens inocultated with CIAV were positive by IIFA 4 days PI,and lasted for at least 6 months. Antibodies, against CIAV were found in chicken flocks in Dalian,Shenyang and Harbin.

关 键 词：鸡病 传染性贫血 病毒 抗体 间接免疫荧光法 

分 类 号：S858.315.3[农业科学—临床兽医学]