小檗碱对豚鼠门静脉平滑肌膜电位及收缩特性的影响  

EFFECTS OF BERBERINE ON MEMBRANE POTENTIAL AND CONTRACTION ACTIVITY OF SMOOTH MUSCLE IN GUINEA PIG PORTAL VEIN

作　　者：黄伟民[1] 徐尚忠[1] 

机构地区：[1]上海市徐汇区中心医院心血管病研究室

出　　处：《中国循环杂志》1995年第2期100-102,共3页Chinese Circulation Journal

摘　　要：用跨膜电位及收缩同步记录方法，观察小檗碱（Ｂｅｒ）对豚鼠门静脉平滑肌细胞膜电位及收缩特性的作用。Ｂｅｒ（２４．５μｍｏｌ／Ｌ）能引起平滑肌细胞膜静息电位较小的去极化，抑制紧张性（ｔｏｎｉｃ）收缩及自发性收缩频率，增加相性（ｐｈａｓｉｃ）收缩幅度。高钾对照组平滑肌细胞膜迅速去极化，紧张性收缩增加，相性收缩消失。Ｂｅｒ能部分对抗Ｋ＋收缩平滑肌的作用。The effects of berberine(Ber) on transmembrane potential and contraction activity were studied with simultaneous recording technique. The resting membrane potential(RMP) of smooth muscle cells in guinea pig portal vein was -48.8±8.0 mV(n=66).Ber (24.5 Pμmol/L) slightly depolarized the RMP from -47.0±2.0 mV to -44. 6±11. 4 mV (n=11, p<0. 05). The tonic contraction and spontaneous contraction frequencies were decreased. The amplitude of phasic contraction was enhanced. 40 mmol/L K+, as a control group, deplorized the RMP from -51.2±10.3 mV to -42.0±10.5 mV (n=9,p<0. 001), and increased the tonic contraction significantly. The phasic contraction was abolished, and the contractive effect induced by K+ was partly reduced by Ber. These results suggested that Ber could cause vasodilation and membrane depolarization.

关 键 词：小檗碱 门静脉 电生理 膜电位 血管平滑肌 

分 类 号：R965.2[医药卫生—药理学]