东亚钳蝎蝎毒二、三级提取物对快反应心肌细胞动作电位的影响  

Influence of the 2nd and 3rd grade abstracts from scorpion venom of Buthus martensii Karsch on action potentials of fast response myocardiocytes

作　　者：江岩[1] 刘伟[1] 钟国赣[2] 杨世杰[2] 张文杰[2] 魏俊杰[3] 张红军[3] 王琳[3] 

机构地区：[1]白求恩医科大学生理中心实验室 [2]白求恩医科大学生理教研室 [3]白求恩医科大学有机化学教研室

出　　处：《中国药理学报》1995年第2期172-174,共3页Acta Pharmacologica Sinica

摘　　要：目的:从东亚钳蝎蝎毒(BMK)中筛选有钠通道阻滞作用的活性成分.方法:培养小鼠心肌细胞,记录快反应动作电位,观察22种BMK蝎毒提取物3 mg·L^(-1)对动作电位的影响,并与河豚毒2.5 mg·L^(-1)、尼莫地平3 mg·L^(-1)、氯化钡24.4 mg·L^(-1)进行对照.结果:BMK-1及其它19种组分使除级化参数V_(max),APA,OS和MDP减小.其作用与河豚毒相似,而与尼莫地平和氯化钡不同.结论:快反应心肌细胞动作电位的改变表明,BMK蝎毒中的20种组分有钠通道阻滞作用.AIM: To screen the active components of sodium channel blockade action from Buthus martensii Karsch (BMK) scorpion venom. METHODS: Myocardiocytes of mice werecultured. Action potentials of fast response myocardiocytes were recorded. The effects of 22 contents of BMK scorpion venom in a concentration of 3 mg.L-1 were tested and compared with that of TTX 2. 5 mg . L-1, ni-modipine (Nim) 3 mg.L-1 and Bad; 24. 4 mg .L-1. RESULTS: BMK-1 and other 19 contents significantly decreased depolarization parameters Vmax, APA, OS, and MDP. which was similar to that of TTX and different from that of Nim and BaCl2. CONCLUSION: The variations in the action potentials of fast response myocardiolcytes indicated that 20 contents of BMK scorpion venom had sodium channel blockade action.

关 键 词：培养的细胞 心肌 动作电位 蝎毒 

分 类 号：R996.3[医药卫生—毒理学]