人参二醇组皂苷Rb_1,Rb_2,Rb_3,R_c和R_d的钙通道阻滞作用和抗自由基作用(英文)  

Calcium channel blockade and anti-free-radical actions of panaxadiol saponins Rb_1, Rb_2,Rb_3, R_c, and R_d

作　　者：钟国赣[1] 孙成文[1] 李云义[1] 齐晖[1] 赵春燕[1] 江岩[2] 王晓明[2] 杨世杰[3] 李红[3] 

机构地区：[1]白求恩医科大学生理教研室 [2]白求恩医科大学生理中心实验室 [3]白求恩医科大学药理教研室

出　　处：《中国药理学报》1995年第3期255-260,共6页Acta Pharmacologica Sinica

摘　　要：目的:确切判定人参二醇组皂苷Rb_1,Rb_2,Rb_3,R_c,R_d的钙通道阻滞作用和抗自由基作用.方法:在Wistar大鼠心室肌细胞上,用连细胞斑片钳技术记录L型、T型、B型单钙通道活动;用电子自旋共振法测定自由基含量.结果:Rb_1,Rb_2,Rb_3,R_c200 μmol·L^(-1)使钙通道的开放时间缩短、关闭时间延长、开放概率减小.30 μmol·L^(-1)拮抗黄嘌呤0.42 mmol·L^(-1)—黄嘌呤氧化酶5.3 nmol·L^(-1)诱发的自由基含量增多,相同剂量的Ra无此二种作用.结论:Rb_1,Rb_2,Rb_2,R_c兼有钙通道阻滞作用和抗自由基作用.AIM: To identify the calcium channel blockade and anti-free-radical actions of panaxadiol saponins Rb1, Rb2, Rb3, Rc, and Rd. METHODS: On ventricular myocardiocytes of Wistar rats, single channel activities of L, T, and B type Ca2+ channels were recorded with the cell-attached configuration of patch-clamp technic, and free radical contents were measured with electron spin resenance method. RESULTS: Rb1, Rb2, Rb3, and Rc 200 unol·L-1 shortened the open times, prolonged the close times, and reduced the open-state probabilities of calcium channels and 30 umol·L-1 antagonized the increase of free radical content induced by xanthine 0. 42 mmol·L-1- xanthine oxidase 5. 3 nmol·L-1. but Rd in the same dose behaved none of the effects. CONCLUSION: Rb1, Rb2, Rb3, and Rc had both the calcium channel blockade and anti-free-radical actions.

关 键 词：钙通道 人参 皂苷类 电子自旋共振谱 自由基 

分 类 号：R965.2[医药卫生—药理学]