甲苯达唑、阿苯达唑和吡喹酮对小鼠细粒棘球蚴囊壁谷胱甘肽硫转移酶和超氧化物歧化酶的作用(英文)  

Effects of mebendazole,albendazole, and praziquantel on glutathione S-transferase and superoxide dismutase of Echinococcus granulosus cyst wall harbored in mice

作　　者：冯建军[1] 郭惠芳[1] 姚民一[1] 肖树华[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心

出　　处：《中国药理学报》1995年第4期297-300,共4页Acta Pharmacologica Sinica

摘　　要：目的:探讨了抗包虫药物对细粒棘球蚴囊壁谷胱甘肽硫转移酶(GST)和超氧化物歧化酶(SOD)的影响,用感染细粒棘球蚴原头节达10—12个月的小鼠作试验.方法:GST用氯硝基苯与还原型谷胱甘肽结合法测定.SOD用改进的邻苯三酚自氧化法测定.结果:囊壁GST和SOD的活力各为12.3±4.3—13.5±4.8 μmol min^(-1)/mg蛋白质,和4.4±2.9—6.1±1.4 U min^(-1)/mg蛋白质.感染鼠用甲苯达唑(Meb)25 mg kg^(-1)d^(-1)治疗14 d时,瘪囊与充盈囊壁的GST活力各被抑制30％和26.8％,而SOD活力则升高105％—163％.阿苯达唑300 mg kg^(-1)d^(-1)和吡喹酮500mgkg^(-1)d^(-1),连给14 d,对上述2种酶无明显影响.结论:Meb抑制囊壁的GST可能损害囊防御系统.To explore the existence of glutathione S-transferase (GST) and superoxide dismu-tase (SOD) in Echinococcus granulosus cyst, and the effect of anthydatid drugs on these 2 enzymes, mice infected with E granulosus pro-toscoleces for 10 - 12 months were used. METHODS: The activity of GST was measured by conjugation rate of l-chloro-2,4-dini-trobenzene with glutathione (reduced form), while the activity of SOD was measured by a modified autoxidation of 1,2, 3-trihydroxy-benzene method. RESULTS: Activities of both GST and SOD in the cyst wall were 12. 3 ±4.3 to 13. 5 it 4.8 umol min-1/mg protein and 4. 4± 2. 9 to 6.1 ±1. 4 U min-1/mg protein, respectively. When infected mice were treated ig with mebendazole (Meb) 25 mg kg-1 d-1 for 14 d, the GST activity of both collapsed and full cyst walls were inhibitied by 30.1 % and 26.8 %, respectively. Whereas SOD activity of the cyst walls were activated by 105 %-163 %. Albendazole 300 mg kg-1 d-1 for 14 d and praziquantel 500 mg kg-1 d-1 for 14 d had no apparent effect on both GST and SOD of E granulosus cyst wall. CONCLUSION:The results suggested that the inhibition of GST activity in the cyst wall induced by Meb might damage the defence system of the parasite.

关 键 词：棘球属 氧化物歧化酶 甲苯达唑 阿苯达唑 

分 类 号：R965.2[医药卫生—药理学]