家兔血小板密度与其聚集和ATP释放反应及细胞内游离钙动员的关系(英文)  

Relationship between platelet density and platelet aggregation, ATP release, and cytosolic-free calcium mobilization in rabbits

作　　者：李柏岩[1] 李文汉[1] 白颖[2] 

机构地区：[1]哈尔滨医科大学药理教研室 [2]哈尔滨市第一医院神经内科

出　　处：《中国药理学报》1995年第4期367-372,共6页Acta Pharmacologica Sinica

摘　　要：目的:研究血小板密度与其功能及单细胞内游离钙动员的关系.方法:以Percoll间断梯度离心法及萤光图像法评价和测量血小板密度及细胞内钙浓度.结果:家兔血小板可分为高(HD),中(ID)和低(LD)三个密度亚群,各亚群之间大小明显差异,且与密度相关(r=0.988,P<0.01).0.5U凝血酶的聚集和ATP释放反应HD均较LD强.150U凝血酶剌激后,单血小板内游离钙水平HD明显高于其它亚群(P<0.01).尽管血清素3μmolL^(-1)无聚集和释放反应,但对细胞内游离钙影响的结果与凝血酶相似.结论:家兔血小板的功能及单细胞内游离钙水平随血小板的密度增加而增强.To study the relationship of platelet density and platelet functions, including aggregation, ATP release, and intracellular cal-cium'mobilization. METHODS: Platelet density was evaluated by discontinuous gradients of Percoll and cytosolic-free calcium concentration in single platelets was measured by fluorescence and imaging. RESULTS: Rabbit platelets were assorted into 3 subpopulationsusing discontinuous gradients of Percoll: high density (HD, >1. 062, 20 %-30 %), intermediate density (ID, around 1.057, 40 % -50%) and, low density (LD, <1.051, 10 % - 15 %). The sizes of platelets in these sub-populations were widely different (HD: 725 ±76; ID: 443 + 47; and LD:307±46) and well correlated with the density (r=0.988, P <0. 01). Thrombin 0.5 U-induced aggregations in the 3 subpopulations were 78±8 %, 69±4 % , and 62±3 % , respectively and a difference was found between HD and LD (P <0. 05). The amounts of ATP released during aggregation were 4. 7 ± 0. 9, 3. 4 ± 1- 0, and 2. 6 ± 0. 6 μmol/4 ×105 platelets in HD, ID, and LD groups, respectively. Thrombin 150 U-induced [Ca2±]i mobilization in HD (990±130 nmol L-1 ) was different (P <0. 01) from those in ID (410± 80 nmol L-1) and LD (400 ± 40 nmol L-1). Serotonin (5-HT) 3μmol L^1 also caused [Ca2±]i mobilization in single-washed platelet subpopulations and results were similar to those of thrombin although it did not induce any detectable aggregation or release reaction. CONCLUSION: Platelet functions and intracellular calcium concentraotion were increased with platelet density in rabbits.

关 键 词：血小板 血小板聚集 腺苷三磷酸 钙 凝血酶 

分 类 号：R965.2[医药卫生—药理学]