粉防己碱对炎症白细胞磷脂酶A_2的作用及其机理探讨  

Effect of tetrandrine on phospholipase A_2 of neutrophiles from inflammatory exudate and exploration of its mechanism

作　　者：何华美[1] 李新芳[1] 张敏[1] 

机构地区：[1]第三军医大学药理教研室,白求恩国际和平医院药械科

出　　处：《中国药理学通报》1995年第1期53-57,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：给大鼠胸膜腔内注射角叉菜胶（１０ｍｇ·ｋｇ－１）复制胸膜炎。粉防己碱（１０～８０ｍｇ·ｋｇ－１）于致炎前３０ｍｉｎ和致炎后４ｈ两次给大鼠ｉｇ能明显抑制炎症白细胞磷脂酶Ａ＿２（ＰＬＡ＿２）和钙调素活性．并降低胞浆内游离钙浓度：在体外，粉防己碱（２０～１６０μｍｏｌ·Ｌ－１）与白细胞悬液孵育３０ｍｉｎ亦能明显抑制炎症白细胞ＰＬＡ＿２活性．这种作用可被Ｃａ￣２＋和钙调素提取液逆转。提示粉防己碱降低炎症白细胞ＰＬＡ＿２活性的作用与其拮抗Ｃａ￣２＋、钙调素系统有关。A pleuritis was induced by an intrapleural injection of carrageenan ( Car, 10 mg· kg-1) in rats. The phospholipase A2 (PLA2) activity, calmodulin activity and cytosolic free calcium concentrationsof neutrophlies from the inflammatory exuate obviously decreased with intragastric administration of tetrandrine( Tet 10～80 mg· kg-1) to rats at 30 min before and 4 h after the injection of Car.Meanwhile.thePLA2 activity of the neutrophiles also decreased when Tet(20～160μmol·L-1) andthe neutrophiles suspension were incubatedtogether for 30 min at 37℃in vitro.The action was reversed in part by calcium and calmodulin extract. These findings suggestthat me inhibition of Tet on me PLA2 activity of neutrophiles may be related to its antagonism on calcium-calmodulin system.

关 键 词：粉防已碱 角叉菜 炎症 磷脂酶A类 钙调素 

分 类 号：R285.5[医药卫生—中药学]