大鼠输精管中蛋白激酶C抑制剂的分离纯化  

Purification of PKC Inhibitor from Smooth Muscle of Mouse Vas Deferens

作　　者：薛海晖 曹颖[1] 杨 陈日新[1] 肖铮[1] 赵冬梅[1] 宗志宏[1] 于秉治[1] 

机构地区：[1]基础医学院生物化学教研室

出　　处：《中国医科大学学报》1995年第5期441-443,共3页Journal of China Medical University

摘　　要：应用凝胶过滤层析及等电聚焦等方法，对大鼠输精管中蛋白激酶Ｃ抑制剂进行了分离纯化。其等电点约在ｐＨ４．５，分子量的为６３ｋＤ。实验表明：大鼠输精管平滑肌中也存在着一种内源性ＰＫＣ抑制剂，并可能与ＰＫＣ共同参与组织细胞生物功能的调控。el filtration chromatography and isoelectrofocusing were used for separation and purification ofPKC inhibitor from smooth muscle of rat vas deferens.The isoelectric point was about 4.5(pH value),molecular weight was about 63 kD.This showed that,the endogenous PKC inhiditor found in bovinespermatozoa also existed in smooth muscle of vas deferens,and may be involved in the regulation of cel-lular biological functions.

关 键 词：蛋白激酶C 抑制剂 大鼠 输精管 分离法 

分 类 号：Q556.3[生物学—生物化学] Q593.9