鼠抗hEGF抗体的制备、活性鉴定及其在免疫组织化学上的应用  

Preparation and Activity Characterization of Antibody Against hEGF in Mice and Its Application in Immunohistochemistry

作　　者：赵宏源[1] 刘玉艳[1] 苏钟浦[1] 王玉玲[1] 

机构地区：[1]中国医科大学基础医学院免进化学研究室,沈阳大学化学系

出　　处：《中国医科大学学报》1995年第5期454-456,共3页Journal of China Medical University

摘　　要：本文利用ｈＥＧＦ作为抗原制备出鼠抗ｈＥＧＦ抗体。该抗体经纯化后用于兔角膜细胞培养，确定其生物学活性作用。通过免疫组织化学上的应用，证明人鳞状皮肤癌组织细胞表面含有ＥＧＦ，并对其应用意义进行了初探。ntibody against hEGF was prepared in mice,In cultured rabbit corneal cell the purified antibodyinhibited the cell growth stimulated by EGF and indicated the biological activity of anti-hEGF.EGF ex-isted in squamous-cell epithelioma was showed by immunohistochemical assay.The results suggest that this research work is useful for clinics.

关 键 词：表皮生长因子 抗体 鼠 免疫组织化学 角膜细胞 

分 类 号：R392.11[医药卫生—免疫学] R329－33[医药卫生—基础医学]