中国啤酒大麦品种RAPD标记的遗传多样性分析  

Genetic Variation Analysis by RAPD of Beer Barley in China

作　　者：张大乐[1] 石永春[1] 李锁平[1] 

机构地区：[1]河南大学生命科学学院,开封475001

出　　处：《武汉植物学研究》2005年第4期305-309,共5页Journal of Wuhan Botanical Research

基　　金：河南省杰出青年科学基金项目(02120000900)资助。

摘　　要：采用RAPD技术对中国38个啤酒大麦品种的遗传资源进行了聚类分析.结果表明:从筛选出的28个有多态性的随机引物中,共扩增出153条谱带,其中91条谱带具有多态性,占59.4%.每个引物可扩增出1～8条多态性谱带,平均3.3条.聚类分析表明,在遗传距离GD值0.27水平上38个啤酒大麦品种可聚成两大类,下分5个亚类.品种间遗传距离GD变幅为0.009 52～0.378 46.RAPD标记揭示出这38个啤酒大麦品种遗传变异较小,遗传基础比较狭窄.The genetic relationships among 38 genotypes of beer barley from China were investigated by RAPD. The results showed that 28 arbitrary primers produced 153 distinctive bands in total and 91 of them were found to be polymorphic, which accounted for 59.4%. Each primer could amplify 1 to 8 polymorphic bands with an average of 3.3 bands. Cluster analysis showed that 38 beer barley could be classified into 2 groups at the level of GD 0. 27,which were respectively classified into 5 subgroups again. The GD varied from 0. 009 52 to 0. 378 46. Most beer barley had showed certain regular distribution in every subgroup. The results indicated that the genetic basis of them was rather narrow.

关 键 词：啤酒大麦 RAPD 遗传多样性 聚类分析 

分 类 号：Q75[生物学—分子生物学] S512.31[农业科学—作物学]