丝素蛋白复合骨髓间充质干细胞构建组织工程化骨修复节段性骨缺损的实验研究  

Application of silk fibroin bio-material combined with bone marrow mesechymal stem cells in the construction of tissue engineered bone for repairing of segmental bone defect

作　　者：苗宗宁[1] 吕国忠 钱寒光 赵基栋 张学光[1] 

机构地区：[1]苏州大学江苏省干细胞研究重点实验室,215007 [2]无锡第三人民医院细胞实验室,214041

出　　处：《国际生物医学工程杂志》2009年第5期273-275,共5页International Journal of Biomedical Engineering

摘　　要：目的 探讨丝素蛋白材料复合骨髓间充质干细胞构建组织工程化骨组织修复兔桡骨的节段性骨缺损的可行性.方法 分离培养兔骨髓间充质干细胞,与丝素蛋白膜材料复合培养,扫描电镜观察细胞在材料上的生长情况.将24只新西兰大白兔制成桡骨中段1.5cm长的骨缺损模型,随机分为3组:实验组(植入细胞材料复合物)、对照组(单纯植入丝素蛋白材料)、空白组(不植入修复材料).术后2,4,8,12周分别行大体观察、组织学观察和X线观察,比较3组骨缺损修复的情况.结果 骨髓间充质干细胞在丝素蛋白膜材料上生长情况良好.术后2,4,8,12周放射学检查新骨生成以及组织学检查新骨生成情况,实验组均优于对照组,空白组各时点均无新骨形成.结论 丝素蛋白材料与骨髓间充质干细胞体外复合培养构建的组织工程化骨能够修复兔桡骨缺损,可以作为组织工程骨的支架材料的全新来源.Objective To investigate the possibility of adopting the silk fibroin biomaterial as the scaf-fold for bone marrow mesechymal stem cells(BMSCs) in the repair of rabbit radial defects. Methods BMSCs were isolated and co-cultured with the silk fibroin biomaterial in vitro. The growth condition of these cells on the scaffold was observed under electronic microscope. Bone defect models were made by removing 15 mm length of midshaft radial bone in 24 New Zealand rabbits. Animals were randomly devided into the experimental group in which the BMSCs seeded silk fibroin scaffold were transplanted, control group in which simple silk fibroin bioma-terial scaffold was adopted and blank group in which rabbits were left untreated. The repair of the defects was observed at 2, 4, 8, 12 weeks after operation, respectively. Results The rabbits BMSCs grew well on the material and the animals treated with grafts exhibited new bone formation. Result from experimental group was apparently superior to that of the control group at 2, 4, 8, 12 weeks after operation. No formation of new bone was found after operation in the blank group. Conclusion The proposed method of combining the BMSCs with silk fibroin bio-material to repair the rabbit radial defects is feasible, and the silk fibroin biomaterial can be applied in the bone tissue engineering as the material of frame.

关 键 词：丝素蛋白 骨髓间充质干细胞 骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]