IgY双抗体夹心ELISA法现场诊断血吸虫病的研究  

Study on IgY immunoglobulin-based double antibody sandwich ELISA for schistosomiaisis diagnosis in the field

作　　者：蒋斌[1,2] 周银娣[1] 孟庆元 罗庆礼[1] 沈继龙[1] 

机构地区：[1]安徽医科大学,安徽合肥市230032 [2]巢湖职业技术学院 [3]当涂县血吸虫防治站

出　　处：《热带病与寄生虫学》2009年第3期138-140,共3页Journal of Tropical Diseases and Parasitology

摘　　要：目的 评价IgY的双抗体夹心ELISA法(S-ELISA)现场诊断血吸虫病的价值.方法 用Lowry法检测包被物的含量,将抗SEA的IgY包被酶标反应板,用棋盘滴定法确定最适抗体包被量以及血清稀释度,建立S-ELISA .以改良加藤厚涂片法 (Kato-Katz法) 检测结果为金标准,采用S-ELISA方法检测血吸虫病流行区部分人群血清循环抗原,并同时用SEA-IHA检测循环抗体,比较两者的敏感性和特异性.结果 检测疫区人群174例,Kato-Katz法、S-ELISA和SEA-IHA阳性率分别是6.90%、22.41%和15.52%,经χ2 检验,P＜0.01,差异有极显著性意义;S-ELISA与SEA-IHA比较,两种方法的敏感性和特异性之间的差异无显著性意义(P＞0.05).结论 S-ELISA诊断血吸虫病具有较好的敏感性和特异性,可以用于疫区筛查血吸虫病.Objective To evaluate the field application value ot lgY immunoglobulin-based double antibody sandwich ELISA for screening on schistosomiasis. Methods Lowry method was used to detect the concentration of coated IgY antibodies against SEA, sera dilution and the optimal amount of antibody-coated were optimized by the method of checkerboard titration, and S-ELISA method was established. Kato-Katz method was used for the gold-standard to confirm schistosomiasis,S-ELISA was used to detect the circulating antigen of schistosome, SEA-IHA was used to detect circulating antibodies and compared with S-ELISA for the sensitivity and specificity. Results Of the 174 inhabitants detected, the positive rate was 6.90%, 22.41%, and 15.52% by the method of Kato-Katz, S-ELISA and SEA-IHA, respectively. There was significant difference for the three methods (P<0.01), and there was no significant difference in sensitivity and specificity between S-ELISA and SEA-IHA (P>0.05). Conclusion S-ELISA has high sensitivity and specificity in the diagnosis of schistosomiasis. It can be used for screening on schistosomiasis in endemic areas.

关 键 词：IGY 双抗体夹心 ELISA法 现场 诊断 血吸虫病 sandwich ELISA 敏感性和特异性 检测结果 改良加藤厚涂片法 显著性 方法 SEA 酶标反应板 疫区人群 循环抗原 循环抗体 血清 差异 Lowry法 

分 类 号：R1[医药卫生—公共卫生与预防医学]