血必净注射液与活化蛋白C对脂多糖诱导大鼠单核细胞组织因子干预效果的比较研究  

A comparison of interference effect between Xuebijing injection (血必净注射液) and activated protein C on lipopolysaccharide-induced tissue factor and protease activated receptor-1 expressions on monocytes in rats

作　　者：归咏刚[1] 姚咏明[2] 柴艳芬[1] 

机构地区：[1]天津医科大学总医院急救中心,天津300052 [2]解放军总医院第一附属医院全军烧伤研究所,北京100048

出　　处：《中国中西医结合急救杂志》2009年第6期326-329,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家重点基础研究发展规划(2005CB522602)，天津市科技创新专项基金(06F22DSH00403)

摘　　要：目的 比较血必净注射液与活化蛋白C(APC)对脂多糖(LPS)刺激大鼠单核细胞表达组织因子(TF)及蛋白酶激活受体1(PAR-1)的影响.方法采用黏附法分离正常大鼠外周血单核细胞后,按随机数字表法分为正常对照组、LPS刺激组、LPS+APC组和LPS+血必净组.分别于LPS刺激后12、24、48、72 h收集细胞,用流式细胞仪检测PAR-1表达的荧光强度;同时留取细胞培养上清液,采用酶联免疫吸附法(ELISA)检测TF、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平.结果 LPS刺激组24 h后各时间点大鼠单核细胞PAR-1的荧光强度较正常对照组显著升高,各时间点上清液中TF、IL-6、TNF-α水平也明显高于正常对照组(P均<0.01).与LPS刺激组比较,LPS+APC组和LPS+血必净组PAR-1、TF、IL-6、TNF-α水平均显著降低(P<0.05或P<0.01);且LPS+血必净组24 h、48 h时PAR-1、TF、TNF-α水平均显著低于LPS+APC组(P<0.05或P<0.01).结论血必净注射液和APC均可显著降低LPS刺激大鼠单核细胞PAR-1表达,减少TF、IL-6、TNF-α分泌,血必净的作用较APC明显.Objective To compare the effects of Xuebijing injection( 血必净注射液) and activated protein C(APC) on expressions of tissue factor (TF) as well as protease activated receptor-1(PAR-1) in monocytes stimulated by lipopolysaccharide(LPS)in vitro．Methods Monocytes were isolated from the blood of healthy Wistar rats by use of adhering method．Monocytes were divided into four groups：normal group，LPS stimulated group，LPS stimulated and APC—treated group，and LPS stimulated and Xuebijing injection—treated group．The monocytes and supernatant were obtained at 12，24，48，and 72 hours after LPS stimulation．The expression of PAR-1 on monocytes was assessed by FACS flow cytometer to determine its intensity of fluore scence，and the levels of TF，interleukin-6(IL-6)and tumor necrosis factor-a (TNF-a) in supernatants were determined by enzyme—linked immunoadsorbent assay( ELISA) ．Results Compared with normal group．the mean fluorescence intensity of monocytic PAR-1 and TF，IL-6，as well as TNF-a levels in the supernatant were significantly increased in LPS stimulated group(all P<0．01) ．In the group with treatment of Xuebijing injection or APC，PAR-1 expression and TF，IL-6，TNF-a levels in supernatants were markedly decreased compared with those in LPS stimulated group( P<0．05 or P<0．01) ．In comparison to LPS stimulation and APC-treated group,PAR-1 expression and TF，TNF-a levels were significantly reduced in LPS stimulation and Xuebijing injection- treated group at 24 hours and 48 hours (P<0.05 or P<O.01).Conclusion Xuebijing injection and APC can markedly down-regulate LPS-stimulated PAR-1 expression on monocytes and TF，IL-6 as well as TNF-a release。and Xuebijing injection might have stronger inhibitory effect than APC in control inflammatory response and coagulative disorder．

关 键 词：血必净注射液 活化蛋白 脂多糖诱导 正常大鼠 单核细胞组织因子 干预效果 比较研究 interference effect comparison tissue factor LPS TNF-α水平 PAR-1 刺激 对照组 APC 荧光强度 IL-6 细胞培养上清液 外周血单核细胞 

分 类 号：R459.7[医药卫生—急诊医学]