雄附散阻抗大鼠肺间质纤维化的病理形态观察  

Pathollmorphological study on Xiongfusan's attenuation of bleomycin-induced pulmonary fibrosis in rats

作　　者：马玉琛[1] 杨永滨[2] 王勇[1,3] 董超[2] 薛娟[2] 

机构地区：[1]解放军第252医院中医康复科,保定071000 [2]河北大学基础医学院形态实验室,保定071002 [3]解放军总医院军医进修学院学员队,北京100853

出　　处：《武警医学》2010年第2期131-134,共5页Medical Journal of the Chinese People's Armed Police Force

基　　金："十一五"军队中医药重大临床攻关课题(2006011003)

摘　　要：目的通过观察肺间质纤维化模型大鼠肺组织的病理形态学变化,探讨雄附散对肺间质纤维化的疗效和早期使用中药干预肺纤维化的意义。方法将70只SD大鼠随机分为正常组、假手术组、模型组、醋酸泼尼松组(5.6mg/kg)、雄附散高、中、低剂量组(1.4、0.7、0.35g/kg)。各组大鼠于造模后的第2天开始连续4周灌胃给予生理盐水(0.014L/kg)或相应药物(0.014L/kg),28d后取右中肺组织进行HE染色和Masson染色及电镜观察。结果 HE染色和Masson染色均提示雄附散高剂量组肺组织无明显纤维化病变,电镜超微结构亦显示雄附散高剂量组肺组织与正常对照组相似。结论高剂量雄附散可有效对抗博来霉素致大鼠肺间质纤维化模型的纤维化过程。Objective To study the effect of Xiongfusan on attenuation of bleomycin-induced pulmonary fibrosis and the value of early-stage intervention by Chinese traditional medicine by observing the pulmonary morphological changes in the model rats. Methods Seventy SD rats were randomly divided into 7 groups(10 rats each),i.e.blank control group,pseudosurgical group,model group,prednisone group(5.6mg/kg),and three Xiongfusan groups(1.4,0.7,0.35 g/kg).Normal saline(0.014 L/kg)and drugs (0.014 L/kg)were administered separately at D_4 post-bleomycin instillation by daily gastrogavage for 4 weeks.The morphologic characteristics (HE stain,Masson stain and ultrastructure)were observed after 28 days treatment.Results No obvious morphologic changes were observed in the high dose Xiongfusan group by histopathologic evaluation,HE staining,Masson staining,or the transmission electron microscopy.Conclusions Xiongfusan may attenuate bleomycin-induced pulmonary fibrosis effectively.

关 键 词：雄附散 肺纤维化 形态病变 超微结构 

分 类 号：R82[医药卫生—临床医学]