单核细胞趋化蛋白-1在肾小球肾炎中的表达及临床意义  

Expression of monocyte chemoattractant protein-1 in human active inflammatory glomerulonephritis and its clinical significance

作　　者：陆海峰[1] 

机构地区：[1]河南省焦作市人民医院肾内科,454000

出　　处：《中国实用医刊》2010年第16期8-10,共3页Chinese Journal of Practical Medicine

摘　　要：目的 观察在急进性肾小球肾炎(RPGN)和Ⅳ型狼疮肾炎(LN)中单核细胞趋化蛋白-1(MCP-I)的表达及临床意义.方法 对20例急进性肾小球肾炎(RPGN)和16例Ⅳ型狼疮肾炎(LN)中肾活检组织进行MCP-1及CD68+浸润细胞免疫组化染色,分析MCP-1在肾小球、肾间质的分布及其与单核巨噬细胞浸润、肾小管间质损伤程度的关系.结果 ①正常组肾脏无MCP-1表达,RPGN和Ⅳ型LN组中大部分患者肾脏MCP-1有表达.②在正常肾组织偶见单核细胞浸润,而在RPGN组和Ⅳ型LN组中见大量单核巨噬细胞弥漫浸润,与正常组比较差异有统计学意义(P<0.01).肾小管MCP-1表达与间质CD68+细胞浸润具有明显相关性.肾小管MCP-1表达越高,MC细胞浸润越明显,肾小管间质损伤就越重.结论 ①在两组病变中,单核细胞趋化蛋白-1都参与了肾小球和肾间质小管的炎症病变;②MCP-1参与原发性肾小球肾炎的发生发展, 针对趋化因子及其受体进行靶向治疗, 阻断白细胞在组织中浸润及其后续反应, 有可能为进展性肾脏疾病的治疗开辟一条新的途径.Objective To investigate the clinical significance of the expression of monocyte chemoattractant protein-1(MCP-I) in rapidly progressive glomerulonephritis(RPGN) and class Ⅳ lupus nephritis(LN). Methods Immunohistochemical method was used to examine MCP-I and MC infiltration in renal biopsy of 20 RPGN,16 class Ⅳ lupus nephritis and 10 normal renal tissue.Results The positive rate of MCP-I and MC infiltration in RPGN and LN was hihger than that in normal renal tissue(P<0.01).The MCP-I had corre1ations with the amount of MC infiltration and tubular interstitial damage.Conclusions The overexpression of MCP-1 in tubular interstitium may attribute to the recruitment of monocytes and thus lead to tubular interstitial damage of human active inflammatory glomerulonephritis. Anti-MCP-I can inhibit inflammation in glomerulonephritis,which might become an effective adjuvant therapy for glomerulonephritis.

关 键 词：单核细胞趋化蛋白 急进性肾小球肾炎 MCP-1表达 临床意义 clinical significance active 单核巨噬细胞浸润 肾小管间质损伤 IMMUNOHISTOCHEMICAL method RENAL tissue 原发性肾小球肾炎 趋化因子及其受体 狼疮肾炎 adjuvant therapy 进展性肾脏疾病 肾活检组织 肾间质 免疫组化染色 RENAL biopsy 统计学意义 

分 类 号：R593[医药卫生—内科学] R692[医药卫生—临床医学]