高致病性猪蓝耳病病毒湖北分离株细胞培养特性研究  

Research on Cell Culture Characteristics of Hubei Strain of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

作　　者：杨克礼[1] 曹宏云[1] 田永祥[1] 梁望旺[1] 伍锐[1] 段正赢[1] 刘泽文[1] 邓均华[1] 郭锐[1] 周丹娜[1] 徐涤平[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所动物胚胎工程及分子育种湖北省重点实验室,湖北武汉430064

出　　处：《江西农业学报》2010年第9期103-106,共4页Acta Agriculturae Jiangxi

基　　金：国家支撑计划项目(2006BAD06A18);湖北省重点实验室开放课题(2010ZD158);湖北省科技攻关计划(2007AA201C36)

摘　　要：高致病性猪蓝耳病是近年来严重危害我国养猪业的一种传染病.为了探明高致病性猪蓝耳病病毒在Marc145细胞上的增殖特性,本研究在分离获得高致病性猪蓝耳病病毒湖北株的基础上进行了细胞培养特性研究.结果表明:高致病性猪蓝耳病病毒湖北株在Marc145细胞上增殖所需的最佳条件分别为:营养液最佳血清含量为10%;最佳pH值为7.2～7.4;细胞最佳培养密度为105～ 2×105/mL;病毒增殖的最佳接毒量为8×103 TCID50/mL;病毒增殖的最佳吸附时间为37 ℃,60 min;最佳收毒时间为接毒后60～84 h;病毒最佳冻融次数为反复冻融3次.The highly pathogenic porcine reproductive and respiratory syndrome was one of the infectious diseases which have been seriously harmful to Chinese pig industry in recent years.In this study,the cell culture characteristics in Marc145 cell of a highly pathogenic strain of porcine reproductive and respiratory syndrome virus(PRRSV) in Hubei province were studied based on the isolation and identification of the Hubei strain.The results showed that the optimum calf serum content,pH-value,density of Marc145 cell,inoculation dose of the strain,adsorption time,harvest time and freezing-thawing times for the proliferation of Hubei strain of PRRSV in Marc145 cell were 10%,7.2~7.4,105~ 2×105/mL,8×103 TCID50/mL,60 min at 37 ℃,60~84 h after its inoculation,and 3 circulations,respectively.

关 键 词：高致病性 猪蓝耳病 病毒增殖 湖北 分离株 细胞培养 特性研究 Culture Characteristics Cell Marc145细胞 最佳条件 增殖特性 严重危害 血清含量 吸附时间 收毒时间 培养密度 反复冻融 冻融次数 营养液 

分 类 号：S[农业科学]