农业害虫铅灰齿爪鳃金龟气味受体基因Or83b的克隆及序列分析  

Molecular Cloning and Sequence Analysis of Odorant Receptor Gene Or83b in Holotrichia plumbea

作　　者：陈全森[1] 姜晓静[2] 鞠倩[2] 赵志强[2] 曲明静[2] 朱新产[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266100 [2]山东省花生研究所,山东青岛266100

出　　处：《江西农业学报》2010年第11期1-4,共4页Acta Agriculturae Jiangxi

基　　金：山东省农业重大应用技术创新课题(鲁财农指[2009]45号);青岛市关键技术攻关项目(08-2-1-25-nsh);山东省自然科学基金项目(2009zrc02070);山东省优秀中青年科学家科研奖励基金(BS2010NY022)

摘　　要：采用简并引物反转录多聚酶链式反应(RT-PCR)和cDNA末端快速扩增(RACE)技术,从农业害虫铅灰齿爪鳃金龟中分离和克隆了一类非常保守的嗅觉受体蛋白基因(命名为Hp-Or83b)全序列。Hp-Or83b全长共1822 bp,5’-UTR为168 bp,3’-UTR为220 bp,开放阅读框全长1434 bp,编码478个氨基酸,预测分子量为54.24 kDa,等电点为7.46。通过同源性比较,发现Hp-Or83b氨基酸序列与已知的其它昆虫的Or83b具有较高的同源性。因此推断所获得的cDNA序列为农业害虫铅灰齿爪鳃金龟气味受体基因Or83b的cDNA序列。An unusually highly conserved olfactory receptor gene(named by Hp-Or83b) was isolated and cloned from agricultural pest Holotrichia plumbea by reverse transcription-polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE) methods.The full-length sequence of Hp-Or83b was 1822 bp.Hp-Or83b contained 168 bp 5'-UTR,220 bp 3'-UTR and a 1434 bp open reading frame which coded 478 amino acids.The protein had molecular mass of 54.24 kDa and isoelectric point of 7.46.The sequence analysis indicated that the deduced amino acid sequences of the cDNA sequences shared high identity with Or83b orthologue receptor sequences from other previously reported insects.So it is inferred that the obtained sequences are cDNA sequences of odorant receptor gene Or83b from agricultural pest H.plumbea.

关 键 词：农业害虫 铅灰 金龟 气味 受体基因 克隆 序列分析 Receptor Sequence Analysis 反转录多聚酶链式反应 CDNA序列 同源性比较 末端快速扩增 氨基酸序列 开放阅读框 嗅觉受体 简并引物 蛋白基因 全序列 分子量 

分 类 号：S[农业科学]