稀土钕(Ⅲ)对水稻线粒体酶活力的影响  

Effect of Rare Earth Nd(Ⅲ) Ion on Activity of Mitochondrial Enzymes in Rice(Oryza sativa)

作　　者：周锡昌[1] 吴明和[2] 杨旻[1] 赵则春[2] 郝广勤 梅启明[1][3] Xi-chang Ming-he Ze-chun Guang-qin Qi-ming 

机构地区：[1]武汉大学,园林中心,湖北,武汉,430072 [2]武汉大学,生命科学学院,湖北,武汉,430072 [3]武汉大学,生命科学学院,湖北,武汉,430072

出　　处：《江西农业学报》2010年第10期17-19,共3页Acta Agriculturae Jiangxi

摘　　要：采用不同浓度(0～60mg/L)的氯化钕(neodymium chloride)对水稻品种进行液体培养,获得线粒体。分析了水稻线粒体抗氧化保护酶及呼吸代谢酶和能量转换酶(COX、SOD、GSH-Px、Ca2+-ATPase、Mg2+-ATPase)活性的变化。结果表明:适当浓度(10～40mg/L)的氯化钕对酶的活力具有明显的诱导和促进作用;但当稀土氯化钕的浓度升高到40～60mg/L时则对酶活力开始产生钝化和抑制作用。分析认为,线粒体抗氧化保护酶及呼吸代谢酶和能量转换酶活性的升高和增强,表明稀土钕可能通过提高线粒体酶的活力,而具有清除含氧自由基和提高作物产品质量的作用。This study used different concentrations(0-60 mg/L) of rare earth neodymium chloride solution to cultivate rice(Oryza sativa) in order to obtain mitochondria.We analyzed the changes in the activity of antioxidative protective enzymes,respiration metabolic enzymes and energy-transferring enzymes(COX,SOD,GSH-Px,Ca2 +-ATPase and Mg2 +-ATPase) in the mitochondria of rice.The results showed that the appropriate concentration(10-40 mg/L) of neodymium chloride had obvious inductive and promotive effects on the activity of the above enzymes,while high concentration(40-60 mg/L) of neodymium chloride had inhibitory action on the enzyme activity.The above results suggested that rare earth Nd(III) ion maybe had the role of removing oxyradicals and improving the quality of crop products through enhancing the activity of the mitochondrial enzymes.

关 键 词：稀土钕 水稻线粒体 线粒体酶活力 RARE Earth Rice 抗氧化保护酶 氯化钕 能量转换 作物产品质量 转换酶 浓度升高 代谢酶 抑制作用 液体培养 氧自由基 水稻品种 酶活性 呼吸 分析 促进作用 

分 类 号：S[农业科学]