高功率毫米波辐照对秀丽线虫行为学和细胞形态学的影响  

Behavioral response and cell morphology changes of Caenorhabditis elegans under high power millimeter wave irradiation

作　　者：任长虹[1,2] 袁广江[3] 高艳[1,2] 吴永红[1,2] 徐志伟[1,2] 徐寿喜[3] 粟亦农[3] 刘濮鲲[3] 张成岗[1,2] 

机构地区：[1]军事医学科学院,放射与辐射医学研究所,蛋白质组学国家重点实验室,北京100850 [2]东南大学,毫米波国家重点实验室,南京210096 [3]中国科学院,电子学研究所,北京100190

出　　处：《强激光与粒子束》2010年第12期2949-2954,共6页High Power Laser and Particle Beams

基　　金：国家自然科学基金项目(30771230);毫米波国家重点实验室开放项目(K200807,K200924);中国博士后科学基金特别资助基金项目(200801495);中国博士后科学基金项目(20060400051)

摘　　要：采用角锥喇叭天线,使用不同输出平均功率的毫米波激光辐照秀丽线虫,采用实时显微摄像系统记录线虫行为学变化,并使用体视显微镜观察线虫细胞形态学变化。受照射后自由活动的秀丽线虫能够快速逃离高功率毫米波的辐照区域。当受输出平均功率为10 W和12 W辐照后,线虫先后出现运动速度增加、运动速度减慢直至身体僵直不动的变化过程。当输出平均功率为5 W时,线虫出现热耐受现象。细胞形态学观察结果显示:辐照后的线虫腹中卵细胞核结构出现不同程度的异常。高功率毫米波辐照可显著影响线虫的行为学特征及细胞形态学,同时说明秀丽线虫是可适用于高功率毫米波生物效应研究的模式生物之一。C. elegans were exposed to high power millimeter waves(MMWs) with different mean power densities, to investigate their behavioral response and cell morphology changes under MMW irradiation. The time-course photomicrography system was used to record the behavioral changes of C. elegans. The behavioral response and cell morphology changes were further observed by stereoscopic microscopes. The results show that freely moving C. elegans will escape from the MMW irradiation region quickly. After the exposure to MMWs with output mean power of 10 W and 12 W, the bending speed of C. elegans increases significantly at first, while the movement gradually slows down until the bodies get rigid. However, exposed to 5 W MMW, C. elegans show a distinctive tolerant reaction because of the thermal effect. In addition, cell morphological observations show that the nuclear structure of the eggs are abnormal after MMW irradiation. High power MMW significantly affects the behaviors and cell morphology of C. elegans, which suggests that C. elegans could be used as a typical model species to study the biological effects of MMW irradiation.

关 键 词：高功率毫米波 激光辐照 秀丽线虫 行为学特征 细胞形态学 millimeter wave high power CAENORHABDITIS elegans changes 平均功率 运动速度 显微摄像系统 输出 角锥喇叭天线 形态学变化 行为学变化 显微镜观察 效应研究 模式生物 观察结果 

分 类 号：O4[理学—物理]