Research on Sex and Tissue Differences in Isozymic Phenotype of Varicorhinus macrolepis through Isozyme Electrophoresis  

泰山赤鳞鱼同工酶性别和组织表型差异的研究(英文)

作　　者：庞秋香[1] 庞书香[2] 赵博生[1] 宿烽[3] 孙欢欢[1] 颜越[1] 吕志林[1] 刘龙[1] 

机构地区：[1]山东理工大学生命科学院,山东淄博255049 [2]济宁职业技术学院生化系,山东济宁272037 [3]青岛科技大学化工学院生物工程与技术系,山东青岛261500

出　　处：《Agricultural Science & Technology》2010年第5期101-105,共5页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(30700071 );Natural Science Foundation of Shandong Province(Y2008D03 );Science and Technology Program of Qingdao City(08-1-27-jch)~~

摘　　要：Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C. 1.1.1.37) isozymes zymogram in 11 different types of tissues of male and female Varicorhinus macrolepis. It had been found for the first time that the phenotype of malate dehydrogenase (MDH),acid phosphatase (ACP) and superoxide dismutase (SOD) showed difference between male and female V. macrolepis,and there was no difference among different individuals in the same sex. Therefore,the electrophoresis band of malate dehydrogenase,acid phosphatase and superoxide dismutase could be used as an indicator for the identification of gender and tissues of V. macrolepis,which would provide basic data for the developmental genetics,variety improvement and directed breeding of V. macrolepis groups,thus facilitating the development and protection of this valuable fish species.应用聚丙烯酰胺凝胶电泳结合生化染色方法分别对雌雄泰山赤鳞鱼11种组织中苹果酸脱氢酶(Malate dehydrogenase,MDH,E.C.1.1.1.37)同工酶酶谱进行了分析。首次发现苹果酸脱氢酶、酸性磷酸酶和超氧化物岐化酶表型在赤鳞鱼雌性和雄性之间存在差异,而在同一性别不同个体之间无差异。因此,苹果酸脱氢酶、酸性磷酸酶和超氧化物岐化酶的电泳酶谱可以作为一项指标用于泰山赤鳞鱼性别和组织的鉴定。这将为赤磷鱼种群的发育遗传学、品种改良和定向育种提供基础资料,从而有利于这一名贵育鱼种的开发和保护。

关 键 词：Varicorhinus macrolepis    Malate dehydrogenase Acid phosphatase Superoxide dismutase Polyacrylamide gel electrophoresis (PAGE) 

分 类 号：S917.4[农业科学—水产科学]