Distribution of Oxytocin Hypothamic-Picuitary-Ovary Axis in Guangxi Local Buffalo  

催产素在广西本地水牛下丘脑-垂体-卵巢轴的免疫组化定位(摘要)(英文)

作　　者：凌泽继[1] 韩涛[1] 李瑞明[1] 秦津[1] 谢莹雪[1] 许典新 杨柄壮[3] 潘琼[4] 奉倩[1] 宋小白[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西区畜禽品种改良站,广西南宁530004 [3]中国农业科学院(广西)水牛研究所,广西南宁530004 [4]西南医院,重庆400038

出　　处：《Agricultural Science & Technology》2010年第5期138-143,154,共7页农业科学与技术（英文版）

基　　金：Supported by Guangxi Scientific Fund Project (Guikezi0991042, Guikezi 0640015 and Guikezi 0832043);Guangxi Area Education Department Educational and Scientific Layout Project (C, 2006C3);Guangxi Education Department Scientific Research Fund (200709LX075);Guangxi Large Apparatus Collaborated Sharing Net~~

摘　　要：To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuperPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo. The test results could provide morphology according to study the OT's synthesis and mechanism of action,and could play reference and directions part in breeding Guangxi local buffalo. The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventromedial nucleus,ventrolateralis nucleus,suprachiasmaticus nucleus,dorsomedial nucleus,mamillary body,anterior hypothalamic nucleus and so on. The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence. In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein cells. The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo. The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.[目的]研究催产素(OT)在广西本当水牛下丘脑、垂体及卵巢的分布上的联系,进而了解OT在下丘脑、垂体及卵巢3者之间分泌释放途径。[方法]采用免疫组织化学SuperPicTureTM二步法检测5头广西本地水牛的下丘脑、垂体和卵巢中催产素(OT)的分布情况。[结果]下丘脑中分泌OT的神经元主要分布在弓状核、视上核及室旁核,在腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑前核等核团也有一定数量的阳性神经元;在腺垂体发现OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部;卵巢中只在生殖上皮、卵泡颗粒细胞和黄体细胞发现有大量OT免疫阳性产物。[结论]首次发现OT存在于广西本地水牛下丘脑-垂体-卵巢轴的各个环节,并且下丘脑中各主要核团均有OT免疫阳性神经元的分布,尤其以弓状核、视上核、室旁核分布最多,为进一步研究OT的合成和作用机制提供形态学依据,并对广西本地水牛的繁殖育种及泌乳起到一定的参考指导作用。

关 键 词：OT    Guangxi local buffalo    Hypothalamuses-pituitary-ovary axis    Immunohistochemistry    SuperPicTureTM two step method 

分 类 号：S823.83[农业科学—畜牧学]