NF-κB在腹部火器伤肠管穿透后肺损伤中的变化及意义  

Expression of pulmonary NF-κB and its significance in lung injury after intestinal perforations due to abdominal firearm wound

作　　者：高伟[1] 刘江伟[1] 张东[1] 胡良煜[1] 冯德元[1] 许永华[1] 邹赛英[1] 

机构地区：[1]解放军兰州军区乌鲁木齐总医院肝胆外科,新疆乌鲁木齐830000

出　　处：《临床军医杂志》2010年第5期683-685,676,共4页Clinical Journal of Medical Officers

基　　金：兰州军区医药卫生科研基金资助项目(LXH-2005019)

摘　　要：目的 探讨NF-κB在腹部火器伤肠管穿透后肺损伤中的变化及意义.方法 健康长白仔猪42头随机等分为对照组和伤后1、 2、 4、 8、 12 h 和24 h 组,实验组建立腹部火器伤肠管穿透模型后,用免疫组化图像分析法测定各组肺组织NF-κB表达,在光镜下观察各组肺脏组织学变化,电镜下观察肺脏超微结构改变.结果 伤后各组肺组织NF-κB表达明显高于对照组,并于伤后1～8 h 内快速上升,在8h 出现高峰(P＜0.05).伤后各组逐渐出现肺泡腔变窄,肺泡壁增厚;肺充血,肺间质水肿.电镜下4、 8、 12、 24 h 组逐渐出现线粒体肿胀、溶解.结论 腹部火器伤肠管穿透后肺组织NF-κB表达活性增强,与肺脏组织病理损伤基本一致,NF-κB在腹部火器伤肠管穿透后继发性肺损伤中具有重要意义.Objective To investigate the expression of pulmonary NF-κB activity and its significance in lung injury after intestinal perforations due to abdominal firearm wound.Methods A total of 42 Changbai piglets were randomized into 7 groups: control group and wounded 1-hour group,2-hour group,4-hour group,8-hour group,12-hour group,and 24-hour group.The model of intestinal perforations due to abdominal firearm wound was established in the wounded groups.Expression of pulmonary NF-κB was measured with immunohistochemical staining and imaging analysis in all the groups.The expression of pulmonary tissue was observed under light microscope and the ultrastructural changes of lung under electron microscope(EMS) in all the groups.Results Expression level of pulmonary NF-κB in the wounded groups was significantly elevated compared to the control group,and with a rapid upgrade from 1-to 8-hour,it appeared to a peak in the 8-hour group(P<0.05).The wounded groups of 4-,8-,12-and 24-hours showed a gradual aggravation such as alveolar space narrowing and wall thickening,pulmonary congestion,interstitial edema,mitochondrial swelling and dissolution under EMS.Conclusion After intestinal perforations due to abdominal firearm wound,pulmonary NF-κB activity increases and it is positively correlative with pulmonary morphological injury.NF-κB may play an important role in subsequent lung injury after the wound.

关 键 词：腹部火器伤 肠管 穿透模型 继发性肺损伤 lung injury 肺组织 镜下观察 组织病理损伤 肺脏 对照组 超微结构改变 表达活性 组织学变化 线粒体肿胀 图像分析法 肺间质水肿 免疫组化 肺泡 电镜 肺充血 

分 类 号：R82[医药卫生—临床医学]