黑斑息肉综合征肿瘤相关基因初步筛选  

Screening and annotation of carcinoma related genes of Peutz-Jeghers syndrome

作　　者：戴益琛[1] 林志翔[1] 姜元芹[1] 陈章兴[1] 谢军培[1] 曾伟[1] 傅育卡[1] 

机构地区：[1]解放军第174医院消化内科,福建厦门361003

出　　处：《临床军医杂志》2010年第5期740-742,共3页Clinical Journal of Medical Officers

基　　金：厦门市科技计划医疗卫生创新项目(3502Z20084031)

摘　　要：目的探索黑斑息肉综合征(PJS)肿瘤特异性相关基因。方法用基因芯片技术研究PJS息肉的基因表达谱,筛选出差异表达基因,建立和研究PJS与肿瘤相关的差异基因表达。结果 大肠PJS息肉411个基因差异表达,其中上调的有197个基因,下调表达的有214个。在基因芯片总共144个癌基因与抑癌基因中,共有13个基因发生差异表达,其中PTTG1、LCN2、LATS2、MADH4、RALB、HRAS、APC、USP4为高表达;IGF2R、ELF3、EGFR、MCAM、ERBB2为低表达。结论这些肿瘤相关基因的差异表达,可能与PJS容易发生恶性肿瘤的潜能有关。Objective To search Peutz-Jeghers syndrome(PJS) carcinoma related genes.Methods Differentially expressed genes of PJS polyp tissues were identified using microarray-based detection.Parts of differentially expressed genes were identified by semiquantitative RT-PCR.Results In a general comparison with normal mucous membranes,411 different expression genes were found in PJS polyps.The carcinoma related genes consisted of PTTG1,LCN2,LATS2,MADH4,RALB,HRAS,APC,USP4,IGF2R,ELF3,EGFR,MCAM and ERBB2.Conclusion The above carcinoma related genes may play important roles in the pathogenesis of PJS polyps.

关 键 词：黑斑息肉 综合 肿瘤相关基因 初步筛选 related genes 基因差异表达 基因芯片技术 差异基因表达 差异表达基因 抑癌基因 肿瘤特异性 基因表达谱 下调表达 恶性肿瘤 PJS息肉 高表达 低表达 ERBB2 潜能 结果 

分 类 号：R82[医药卫生—临床医学]