寒冷高湿环境下犬肢体爆炸伤伤口细菌学变化观察  

Bacteriology in explosive wounds of canine limbs in cold and humid environment

作　　者：沈开金[1] 刘正祥[2] 吉善和[1] 李新宇[1] 张培谊[3] 雷权[4] 吴顺久[4] 许晴[5] 

机构地区：[1]解放军兰州军区乌鲁木齐总医院急诊科,新疆乌鲁木齐830000 [2]解放军兰州军区乌鲁木齐总医院检验科,新疆乌鲁木齐830000 [3]解放军兰州军区乌鲁木齐总医院病理科,新疆乌鲁木齐830000 [4]解放军兰州军区乌鲁木齐总医院医务部,新疆乌鲁木齐830000 [5]解放军兰州军区乌鲁木齐总医院实验动物科,新疆乌鲁木齐830000

出　　处：《临床军医杂志》2010年第6期887-889,共3页Clinical Journal of Medical Officers

基　　金：兰州军区乌鲁木齐总医院重点资助课题(No:ZH01-09)

摘　　要：目的实验研究寒冷高湿环境下杂种犬肢体爆炸伤伤口细菌学变化,为战伤救治提供依据。方法建立寒冷高湿环境杂种犬肢体爆炸伤模型,犬雷管炸伤肢体后,于伤后即刻、4、6、8、12、24、48、72、96 h取伤口深处组织行细菌培养,周围空气、物品表面、实验犬皮毛拭洗液采样细菌培养、定量检测,定性分析和药敏试验。结果寒冷高湿环境杂种犬肢体爆炸伤后,伤口组织培养有细菌生长,主要菌群为革兰阳性菌,菌群随时间延长移位、改变,伤口感染时间为伤后24 h。结论寒冷高湿环境下伤口感染时间为伤后24 h,研究结果为早期治疗提供理论依据。Objective To investigate the early infection characteristics of canine limbs following explosive injury in cold and humid environment,so as to provide basis for early treatment of combat wound.Methods The model of canine limbs exploded was established in cold and humid environment.Deep wounded soft tissue of was adopted immediately after explosion and at 4-,6-,8-,12-,24-,48-,72-,and 96-h,respectively.Deep wounded tissue,vein bleed,canine fur cleansing solution,air and surrounding item surface at explosion range were cultured,and the quantitative bacteria and drug susceptibility were analyzed.Results After canine limbs were exploded in the cold and humid environment,wound inflammatory change was significant at 24-hour.Tissue and vein bleed culture showed bacterial growth and wound infection.G+ bacteria were most of them,the flora changed following the time.Conclusion In cold environment with high humidity,the wounds are infected during a period of 24 hours,which provides a theoretical basis for treatment of war wound.

关 键 词：高湿环境 肢体爆炸伤 伤口感染 细菌学 杂种犬 细菌培养 时间 革兰阳性菌 组织培养 周围空气 战伤救治 早期治疗 药敏试验 细菌生长 实验研究 理论依据 菌群 结果 定性分析 定量检测 

分 类 号：R82[医药卫生—临床医学]