以四磺基铝酞菁-牛血清白蛋白为底物的荧光恢复均相法测定胃蛋白酶  

A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate

作　　者：陶云[1,2] 黄萍[2] 邓雅斌[2] 李东辉[2] 

机构地区：[1]厦门大学生命科学学院 [2]厦门大学医学院抗癌研究中心,厦门361005

出　　处：《分析化学》2010年第12期1781-1784,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20015005);福建省科技项目(No.99H048)资助

摘　　要：酸性介质中,红区荧光探针四磺基铝酞菁(AlS4Pc)的荧光被白蛋白显著猝灭,加入胃蛋白酶后,体系荧光明显回复。基于此现象,建立了荧光恢复均相测定胃蛋白酶的新方法。考察了各种影响因素,在最佳实验条件(pH2.5、反应温度50℃、反应时间1h)下,本方法的线性范围为0.04～4mg/L,检出限为20μg/L。用本方法测定实际样品中胃蛋白酶,取得了满意的结果。It was found that the fluorescence of aluminium tetrasulphonated phthalocyanine(AlS4Pc),a redregion fluorescence probe,was extremely quenched by bovine serum albumin(BSA) in acidic media,but recovered dramatically after the addition of pepsin.Based on this phenomenon,a new method was developed for homogeneous determination of pepsin.Under the optimal conditions(pH 2.5,reaction temperature 50 ℃ and reaction time of 1 h),the linear range of assay was 0.04-4 mg/L and the detection limit was 20 μg/L.The constructed method has been used to the analysis of pepsin tablet samples with satisfied results.

关 键 词：荧光探针 酞菁 胃蛋白酶 荧光恢复 红外区荧光 

分 类 号：R341[医药卫生—基础医学]