急性酒精中毒的鼠脑内乙醛及其单胺神经递质缩合物的测定  

Determination of Acetaldehyde,Salsolinol and 6-Hydroxy-1-methyl1,2,3,4-tetrahydro-β-carboline in Brains after Acute Ethanol Administration to Neonatal Rats

作　　者：毛健[1,2] 徐妍[1] 邓玉林[1] 林凡凯[1] 谢冰洁[1] 王睿[1] 

机构地区：[1]北京理工大学生命学院,北京100081 [2]中国烟草总公司郑州烟草研究院,郑州450001

出　　处：《分析化学》2010年第12期1789-1792,共4页Chinese Journal of Analytical Chemistry

基　　金：北京市科技计划(No.20070639034)资助项目

摘　　要：应用高效液相色谱法测定了急性酒精中毒后新生鼠脑中乙醛的含量,以及乙醛分别与多巴胺、5-羟色胺的缩合产物1-甲基-6,7-二羟基-1,2,3,4-四氢异喹啉(Salsolinol,Sal)和6-羟基-1-甲基-1,2,3,4-四氢-β-咔啉(6-OH-MTHβC)的含量。采用2,4-二硝基苯肼(DNPH)的乙腈溶液在60℃水浴中衍生15min提取脑匀浆中的乙醛,离心后进行色谱分离,检测波长为360nm,本方法简单准确,灵敏度高。应用本方法测定鼠脑组织中的乙醛含量时发现,急性酒精中毒后新生鼠脑内乙醛的含量在24h内显著高于盐水对照组(P<0.05)。同时,采用HSF5柱分离和库仑阵列电化学检测的方法对Sal和6-OH-MTHβC进行分析表明,乙醛浓度升高后,急性酒精中毒鼠脑内Sal和6-OH-MTHβC的含量显著升高(P<0.05)。乙醛的非正常代谢及内源性神经毒素的生成可能在酒精的神经毒性机理中发挥了重要作用。Acetaldehyde,salsolinol(Sal) and 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline(6-OHMTHβC) in neonatal rat brain after acute ethanol treatment were determined by high performance liquid chromatography.Free acetaldehyde in rat brain homogenate was derivatized with 2,4-dinitrophenylhydrazine(2,4DNPH) at 60 ℃ for 15 min.The derivate product was separated on an ODS-C18 column(4.6 mm × 150 mm) and detected at 360 nm.The method was proved to be simple,sensitive and reliable.In comparison with the control,analysis of neonatal rat brain samples revealed a significant difference in the level of acetaldehyde within 24 h of 5.0 g/kg ethanol treatment(P 0.05).The determination of Sal and 6-OH-MTHβC by HPLC was performed on a HS F5 column(250 mm × 4.6 mm),with a Coularray Detector-4 as the electrochemical detector.The results indicated that the levels of Sal and 6-OH-MTHβC in rat brain increased significantly with the elevated level of acetaldehyde after acute alcohol administration.The condensation of acetaldehyde with monoamine neurotransmitters may play an important role in central nervous system dysfunction after alcoholism.

关 键 词：急性酒精中毒 鼠脑 乙醛含量 单胺神经递质 缩合物 液相色谱法测定 内源性神经毒素 中毒后 神经毒性机理 方法 四氢异喹啉 二硝基苯肼 电化学检测 Sal 正常代谢 新生 浓度升高 色谱分离 羟基 灵敏度高 

分 类 号：R595.6[医药卫生—内科学]