紫叶加拿大紫荆组织培养研究  

Tissue Culture of Cercis canadensis Forest Pansy

作　　者：孟月娥[1] 李艳敏[1] 赵秀山[1] 王利民[1] 王慧娟[1] 董晓宇[1] 

机构地区：[1]河南省农业科学院园艺研究所,河南郑州450002

出　　处：《河南农业科学》2010年第12期114-117,共4页Journal of Henan Agricultural Sciences

基　　金：河南省发改委项目(2060403)

摘　　要：研究了外植体采集时间,不同质量浓度的6-BA、NAA、蔗糖以及不同部位茎段对紫叶加拿大紫荆增殖生长的影响,并初步研究了基本培养基I、BA、NAA对生根的影响。结果表明,紫叶加拿大紫荆外植体最佳的采集时期为5月;其增殖培养基为WPM+6-BA 1.5～2.5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L,增殖系数最高达3.3;带愈伤组织的下部茎段的增殖效果好于不带愈伤组织上部茎段,上部茎段在高质量浓度的6-BA中增殖效果好;基本培养基、IBA质量浓度、NAA质量浓度对生根率有影响,最佳的配比是MS+IBA 5 mg/L+NAA 0.5 mg/L。The different stem segments of Cercis canadensis Forest Pansy(Leguminosae) were taken as explants to investigate the effects of different hormone combinations in the basic culture medium to the multiplication and rooting.The results showed that May was the optimum period for explant collection,the suitable medium for multiplication was WPM+6-BA(1.5-2.5mg/L)+NAA(0.1mg/L)+sugar(30g/L),and the highest propagation coefficient could reach to 3.3;the lower stem segments with callus tissue were better than the upper ones without callus tissue for multiplication,the latter needed higher concentration of 6-BA for multiplication;the best hormone combination for inducting rooting was MS+5mg/L IBA+0.5mg/L NAA.

关 键 词：紫叶加拿大紫荆 愈伤组织 培养研究 高质量浓度 基本培养基 增殖效果 茎段 NAA 外植体 6-BA 增殖培养基 IBA 蔗糖 增殖系数 增殖生长 生根率 上部 初步研究 采集时间 同部位 

分 类 号：S[农业科学]