脉冲电化学沉积法制备钛基HA/Ag复合涂层  

Preparation of HA/Ag Composite Coatings by Pulse Electrochemical Deposition on Titanium Substrate

作　　者：张柏林 王英波 鲁雄 周先礼 屈树新 冯波 翁杰 

机构地区：[1]SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China

出　　处：《稀有金属材料与工程》2010年第10期1835-1839,共5页Rare Metal Materials and Engineering

基　　金：国家自然科学基金(30700172);高等学校博士学科点专项科研(新教师)基金(20070613019);国家科技支撑计划(2006BAI16B01)

摘　　要：采用脉冲电化学法在生物医用钛表面制备出纳米HA/Ag复合涂层。借助SEM、XRD等对复合涂层的形貌、成分进行表征。利用大肠杆菌和白色葡萄球菌两个菌种对该涂层抗菌性能进行分析研究。最后利用MC3T3-E1成骨细胞检测复合涂层的细胞相容性。结果表明:HA/Ag复合涂层呈纳米球状,由HA和Ag两相组成。Ag在HA涂层中分布均匀。复合涂层对两种细菌均有较好的抗菌效果,对大肠杆菌抗菌率达100%,对白色葡萄球菌的抗菌率接近100%,对大肠杆菌的抗菌能力高于白色葡萄球菌。细胞培养实验表明MC3T3-E1成骨细胞在HA/Ag复合涂层表面的黏附、铺展较好。综合可知,脉冲电化学沉积法制备的纳米HA/Ag复合涂层具有较好的抗菌性和细胞相容性。Uniform and dense nano-spherical HA/Ag composite coatings were prepared by a pulse electrochemical deposition method on biomedical titanium surfaces. The coatings were characterized by XRD and SEM. Escherichia coli and Staphylococcus albus were utilized to test the antibacterial ability of the coatings. The cytocompatibility of composite coatings was evaluated by culturing osteoblast MC3T3-E1 on the coatings. The results demonstrate that Ag uniformly distribute in the composite coatings without agglomeration. The coatings have good antibacterial effects on the two types of bacteria. However, they have a better antibacterial effect on Escherichia coli of 100% than on Staphylococcus albus of near 100%. The MC3T3-E 1 cell culturing results indicate that the MC3T3-E1 osteoblast has good adhesion and spreading on HA/Ag composite coatings. It is concluded that HA/Ag composite coatings prepared by pulse electrochemical deposition have good antibacterial property and cytocompatibility.

关 键 词：脉冲 电化学沉积 法制备 钛基 复合涂层 Electrochemical Deposition 白色葡萄球菌 大肠杆菌 细胞相容性 成骨细胞 MC3T3-E1 纳米 抗菌性能 抗菌率 细胞检测 涂层表面 培养实验 抗菌效果 抗菌能力 分析研究 

分 类 号：R318.08[医药卫生—生物医学工程]