幽门螺杆菌抗原检测的临床诊断价值  

Evaluation of the ELISA Method for the Determination of the Helicobacter Pylor Stool Antigen

作　　者：孙艳艳[1] 吴雪梅[1] 

机构地区：[1]北京市石景山医院检验科,100043

出　　处：《慢性病学杂志》2010年第12期1644-1645,共2页Chronic Pathematology Journal

摘　　要：目的探讨粪便幽门螺杆菌抗原(Hp stool antigen,HpSA)的临床诊断价值。方法采用酶联免疫吸附试验(ELISA)检测94例因上消化道症状接受胃镜检查患者的幽门螺杆菌粪便抗原。每例患者同时进行14C-UBT、RUT、Warthin-Starry银染检测,将后三项中任两项阳性作为Hp感染阳性诊断标准,对HpSA方法进行评价。结果 HpSA检测的敏感性为94.3%,特异性为90.2%,准确性为92.6%,阳性预测值为92.6%,阴性预测值为92.5%。结论 HpSA检测的敏感性、特异性和准确性较高,具有简单可靠、标本留取方便及完全非侵入性的特点,适合作为Hp感染的常规检查项目。Objective To research the clinical diagnosis value of the Helicobacter pylor stool antigen (HpSA)test.Methods Stool specimens were collected from 94 patients who had gastroscopy for gastrointestinal symptoms.Enzyme-linked immunosorbent assay(ELISA)was used to determinat Helicobacter pylor antigen in the stool.Hp status was evaluated by three different tests:14C-UBT、RUT、Warthin-Starry.Any two of them were positive are regared Hp infection.Results The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of HpSA test were 94.3%,90.2%,92.6%,92.6%,92.5% respectively.Conclusion HpSA has highly sensitivity and specificity,it is a simple,reliable,easy to gain speciman,non-invasive and may be used as a routine test of Hp infection in clinical laboratory.

关 键 词：螺杆菌 幽门/免疫学 抗原 细菌/分析 人类 

分 类 号：R446.6[医药卫生—诊断学]