RP-HPLC法测定烟叶提取物中茄尼醇的含量  

RP-HPLC Determination of Solanesol in the Extracts of Tobacco Leaves

作　　者：叶才秋[1] 邓军[2] 龚文[2] 祝春光[2] 方泽银[2] 白琦[2] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014 [2]四川农业大学食品学院,四川雅安625014

出　　处：《安徽农业科学》2010年第36期20633-20634,20636,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立测定烟叶提取物(粗品、精品和纯品)中茄尼醇含量的方法。[方法]用甲醇提取烟叶提取物中的茄尼醇。高效液相色谱条件:Kromasil5-C18分离柱(150.0 mm×4.6 mm,5.0μm),流动相为甲醇-乙醇(30∶70),流速为1.3 ml/min,紫外检测波长213 nm。[结果]茄尼醇在0.1～1.5 mg/ml线性关系良好,相关系数0.999 5,最低检测量为0.000 1 mg,有良好的分离效果和回收率(97.2%)。[结论]该方法操作简便快速,有良好的精密度和重现性,可用于测定烟叶提取物茄尼醇的含量。[Objective] To develop an HPLC method for the determination of solanesol in the extracts of tobacco.[Method] Solanesol in tobacco leaves extract was extracted with methanol.The separation was performed on a Kromasil 5-C18 Column(150.0 mm×4.6 mm,5.0 μm),with the mobile phase of Ethanol-methanol(30∶70).The flow rate was 1.3 ml/min and the detection wavelength was 213 nm.[Result] Solanesol have good linear relationship in the range of 0.1-1.5 mg/ml(r=0.999 5).The lowest detection concentration was 0.000 1 mg.T...

关 键 词：茄尼醇 含量测定 RP-HPLC 

分 类 号：S572[农业科学—烟草工业]