利用磁珠富集法筛选大银鱼微卫星标记的初步研究  

Isolation of Microsatellite Loci Using magnetic-bead Enrichment Protocol in Protosalanx Hyalocranius

作　　者：赵亮[1] 高贵珍[1] 张兴桃[1] 

机构地区：[1]宿州学院化学与生命科学学院,安徽宿州234000

出　　处：《宿州学院学报》2010年第11期24-26,53,共4页Journal of Suzhou University

基　　金：宿州学院硕士启动基金项目(2007YSS10);安徽省教育厅自然科学重点项目(KJ2009A052Z)

摘　　要：微卫星基因位点是在遗传学各个领域被广泛使用的分子标记,但对于首次研究的物种,必须筛选出可以应用的微卫星位点。为促进大银鱼保护遗传学的开展,采用磁珠富集微卫星序列技术,提高了微卫星位点的筛选效率,成功获得6个具有多态性的(CA)n重复序列的大银鱼微卫星位点。Microsatellite loci has become one of the most popular molecular markers used in many genetics fields.However,it is crucial that polymorphic microsatellite loci needs to be selected or isolated for the studied species at first.In order to promote the genetic conservation in Protosalanx hyalocranius,we have isolated and characterized 6 polymorphic(CA)n microsatellite loci using "magnetic-bead enrichment" protocol in this species,and these loci of high variability and stability will be useful in population ge...

关 键 词：微卫星 基因组文库 筛选 大银鱼 

分 类 号：Q75[生物学—分子生物学]