资木瓜皂苷提取工艺的优化及对大鼠佐剂性关节炎抗炎免疫机制的研究  

Optimization on extraction technology of saponin of chaenomeles speciosa and study on its anti-inflammatory immunologic mechanism to rat adjuvant arthritis

作　　者：杨兴海[1,2] 吴杰[1] 郭莲军[3] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]天然产物研究与利用湖北省重点实验室,湖北宜昌443002 [3]华中科技大学同济医学院,湖北武汉430030

出　　处：《华西药学杂志》2010年第5期547-549,共3页West China Journal of Pharmaceutical Sciences

基　　金：天然产物研究与利用湖北省重点实验室资助项目(No2008NP02)

摘　　要：目的研究资木瓜皂苷(SCS)提取工艺的优化及对大鼠佐剂性关节炎(AA)的抗炎作用及免疫机制。方法以SCS的提取总量为考察指标,采取正交试验法考察乙醇浓度、溶媒用量、提取时间、提取次数等因素,每个因素设置3个水平。流式细胞术检测SCS对AA外周血中CD4+、CD8+的表达水平;MTT法检测SCS对AA外周T淋巴细胞增殖的影响。结果 SCS最佳提取工艺为乙醇浓度70%,溶媒容量10倍,提取时间1h,提取3次,提取率为1.7%。SCS能降低AA外周血中CD4+的表达水平,对CD8+的表达无明显影响,对AA外周T淋巴细胞增殖有抑制作用。结论最佳提取条件为A2B1C2D3,SCS通过抑制T淋巴细胞增殖和降低CD4+表达而发挥抗炎免疫作用。OBJECTIVE To study optimization on extraction technology of saponin of Chaenomeles Speciosa( SCS) and study on its anti-inflammatory immunologic mechanism to rats adjuvant arthritis( AA) . METHODS Orthogonal design L9 ( 34 ) on four factors including alcohol level,alcohol volume,extraction time and extraction frequency was used,and took the saponin rate as the index. MTT was used to determine lymphocytes proliferative activity. T lymphocytes subgroup CD4 + and CD8 + in peripheral blood were detected by flow...

关 键 词：木瓜皂苷 提取工艺 正交试验 淋巴细胞增殖 佐剂性关节炎 抗炎免疫 

分 类 号：R284.2[医药卫生—中药学]