苦瓜籽α-苦瓜素的分离纯化及其生物活性的研究  

Preparation of α-momorcharins from Momordica charantia and study on biological activity

作　　者：刘盛邦[1] 吴民沪[2] 黄文虎[1] 杨梅强[1] 邓缅[1] 姚兴川[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都610064 [2]成都医学院,四川成都610083

出　　处：《华西药学杂志》2010年第6期700-702,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金(批准号:30770232)

摘　　要：目的建立一种快速、高效制备α-苦瓜素的方法 ,并初步研究其部分生物活性。方法用硫酸铵分级沉淀、SP-Sepharose FF离子交换层析、Sephacryl S-100分子筛层析以及Macrocap SP离子交换层析等方法 ,从苦瓜籽中纯化出苦瓜蛋白(α-MMC);通过MTT实验,研究了α-MMC的抗肿瘤活性;测定HepG2.2.15 cell的培养上清液中HBsAg和HBeAg的含量,研究了α-MMC的抗HBV活性。结果与结论α-MMC具有抑制HBV和肿瘤细胞增殖的作用,呈时效和量效依赖性。OBJECTIVE To establish a rapid and efficient method for preparation of α-MMC and study preliminarily its biological activity.METHODS Alpha-momorcharins(α-MMC) was isolated and purified from the seeds of bitter melon by ammonium sulphate precipitation,chromatographies on SP-Sepharose FF,Sephacryl S-100 and Macrocap SP.The anti-tumor activity of α-MMC was studied by MTT assay.The anti-virus activity of α-MMC was studied by measuring the contents the HBsAg and HBeAg in cultured medium of HepG2.2.15 cell.RESULT...

关 键 词：苦瓜籽 α-苦瓜素 分离纯化 抗肿瘤活性 抗病毒活性 

分 类 号：R285[医药卫生—中药学]