LC-MS测定两种来源续断中的川续断皂苷Ⅵ  

Qualitative and quantitative comparison with asperosaponin Ⅵ of Dipsacus asperoides from different locations by LC-MS

作　　者：吴春蕾[1] 李厚聪 张志锋[1] 徐彦[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041 [2]湖北民族学院医学院,湖北恩施444500

出　　处：《华西药学杂志》2010年第6期738-740,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技厅应用基础研究项目(2009JY0017)

摘　　要：目的测定两种不同来源续断药材中不同部位的川续断皂苷Ⅵ。方法采用LC-MS法和质谱法定性定量测定川续断皂苷Ⅵ的含量,选用Eclipse XDB C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水溶液(30:70),流速1.0 mL.min-1,检测波长210 nm;并用ESI-MS定性分析川续断皂苷Ⅵ。结果川续断皂苷Ⅵ进样量0.204~2.04μg与峰面积的线性关系良好(r=0.9992);平均回收率为99.92%,RSD=1.31%。川续断药材中根、茎、叶中均含有川续断皂苷Ⅵ,含量分别为2.48%、0.60%、0.50%;而鹤峰续断只有根中含有川续断皂苷Ⅵ,含量为2.42%。结论所建方法简便可行、专属性强,可控制续断药材及其制剂的质量。两种来源的续断植物在茎、叶中的含量差异可为川续断属植物提供化学分类学依据。OBJECTIVE To determine the contents of asperosaponin Ⅵ in Dipsacus asperoides from two different locations and different parts.METHODS HPLC-MS was adopted to analyze the content of Asperosaponin Ⅵ.The HPLC system consisted of Eclipse XDBC18(150 mm×4.6 mm,5 μm) column with acetonitrile-water(30:70) mixture as mobile phase and flow rate of 1.0 mL·min-1.RESULTS The average recovery was 99.92 % and RSD was 1.31%.The content of asperosaponin Ⅵ in Dipacus asperoides from Kangding county were as follows:2.48% in r...

关 键 词：川续断 川续断皂苷Ⅵ 液质联用技术 

分 类 号：R284[医药卫生—中药学]