粪便幽门螺杆菌抗原检测与胃黏膜病的关系研究  

Study of the relationship of the antigen test of the helicobacter pylori in stool and the gastric mucosal pathology

作　　者：朱桂峰[1] 张风云[1] 荣秋华[1] 金红[1] 高恒强[1] 

机构地区：[1]山东省聊城市人民医院检验科,山东252000

出　　处：《医学检验与临床》2006年第1期33-35,共3页Medical Laboratory Science and Clinics

摘　　要：目的探讨酶免疫法(ELISA)检测幽门螺杆菌(Hpylori)粪便抗原的特异性及敏感性。方法用酶免疫试剂对90例接受胃镜检查并取活检组织的患者粪便标本进行HP粪便抗原检测,确认试验为胃粘膜组织HP培养和尿素酶试纸快速试验检查,若结果均阳性则确认为HP感染。将粪便抗原检查结果与确认试验的结果比较,判断其敏感性、特异性和准确性。结果90例患者中确认为HP阳性的54例,阳性率为60%,其中消化性溃疡、胃炎、胃癌的阳性率分别为63.3%、64.7%、50.0%。HP粪便抗原阳性的57例患者中HP阳性的54例;HP阴的36例患者中HP粪便抗原阴性的29例,其敏感度为94.7%、特异度为83.7%。结论酶免疫法检测HP粪便抗原,方法可靠,易于推广。Objective To evaluate the sopecificity and sensitivity of a enzyme lmmunoassay for detection of Helicobacter pylori stool antigenHPSA).Methods Stool specimens of 90 patients who received gastroscope examination and biopsy were detected with enzymeimmunoassay.It was confirmed a diagnostic test of H Pylori state was defined as positive when two tests were positive.The sensitivity and specificity were evaluated while comparing the results of the H Pylori stool antigen with that results of confirmed diagnosis test.Resrlts H Pyloripositive rate was 60.0%.The positive rate of peptic ulcer, gastritis and gastric carcinoma were 63.3%、64.7%、50.0%.respectively The patients with the positive H Pylori stool antigen54cases) were diagnosed as H Pylori infection,the patients with the negative H Pylori stool antigen29cases) were confirmed negative patients.the sensitivity was 94.7%54/57).The specificity was 83.7%29/34). Conclusion Enzyme immune assay for detecting the H Pylori stool antigen is a simple,accurate direct method and it is easy to be extened.

关 键 词：粪便幽门螺杆菌 粪便抗原检测 黏膜病 关系研究 阳性率 确认试验 酶免疫法 患者 特异性 敏感性 胃镜检查 消化性溃疡 胃粘膜组织 酶免疫试剂 快速试验 抗原阳性 结果比较 检查结果 活检组织 粪便标本 

分 类 号：R446.6[医药卫生—诊断学]