ELISA和TRUST法在梅毒检测中的应用价值  

Study of the Value of TRUST and ELISA methods in the detection of treponema pallidum

作　　者：姚家奎[1] 顾蓉[2] 冯霞[2] 王华明[2] 卢秋香[3] 

机构地区：[1]南京医科大学 [2]江苏省苏北人民医院检验科 [3]安徽省蚌埠医学院

出　　处：《医学检验与临床》2006年第2期13-14,共2页Medical Laboratory Science and Clinics

摘　　要：目的探讨梅毒酶联免疫吸附试验(ELISA)、甲苯胺红不加热血清试验(TRUST)在梅毒诊断中的应用价值。方法应用TRUST、ELISA法分别检测2862例患者血清;梅毒螺旋体明胶颗粒凝集试验(TPPA)检测的43例患者与ELLSA、TRUST结果进行了对比观察。结果ELISA法检出率为1.61%(46/2862);TRUST法检出率为1.11%(32/2862),两者之间的总符合率为99.13%(2840/2862);ELISA法假阳性、假阴性率分别为0.06%(l/17)、7.69%(22/26);TRUST法假阳性、假阴性率分别为29.41%(5/17)、57.69%(15/26)。结论在输血前、疑似梅毒血清学检查中两者联合检测具有重要临床价值。Objective To discuss the value of TRUST and ELISA in anti-TP Methods TRUST and TP-ELISA,wcre used to detect the serum specimens from 2816 patients;TPPA method was used to detect the serum specimens from 43 patients.Then TPPA method was employed to confirm the results of ELISA and TRUST.Results The rate of ELISA was 1.61%(46/2862) and The rate of TRUST was 1.11%(32/2862),The total coincidental rate between ELISA and TRUST was 99.13%(2840/2862);The falsc positive and false negative of ELISA were 0.06%(l/17) and 7.69%(2/26);The false positive and false negative of TRUST were 29.41%(5/17) and 57.69%(15/26).Conclusion It is suggested that the approach of TRUST and ELISA should join up to test the anti-TP in order to have important clinical value before blood transfusion or detecting borderline case.

关 键 词：梅毒检测 应用价值 明胶颗粒凝集试验 酶联免疫吸附试验 假阴性率 梅毒血清学检查 检出率 假阳性 梅毒螺旋体 血清试验 梅毒诊断 临床价值 联合检测 甲苯胺红 患者血清 对比观察 输血前 符合率 不加热 方法 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]