羟基磷灰石/β-磷酸三钙/甲壳素复合多孔支架材料的制备及生物相容性研究  

The Preparation And Biocompatibility Of The Porous Hydroxyapatite/Beta -Tricalcium Phosphate/Chitin Scaffold Material

作　　者：王红梅[1] 陈庆华[1] 潘兴华[2] 庞荣清[2] 丑修建[1] 

机构地区：[1]昆明理工大学材料与冶金工程学院,云南省昆明市650093 [2]成都军区昆明总医院,云南省昆明市650032

出　　处：《组织工程与重建外科杂志》2006年第1期21-24,共3页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：云南省二层次人才培养项目(2000YP13;20034)

摘　　要：目的探讨羟基磷灰石/β-磷酸三钙/甲壳素(HAP/β-TCP/CS)多孔支架的制备及其与人体骨髓间质干细胞(MSCs)的生物相容性。方法以羟基磷灰石粉体为基体,采用有机泡沫浸渍-发泡复合成型工艺制备出羟基磷灰石/β-磷酸三钙多孔支架,再将该多孔支架与甲壳素复合得到有机-无机复合支架,然后通过体外培养实验评价该复合支架的干细胞相容性。结果复合甲壳素可以显著提高HAP/β-TCP支架的抗压强度,并且该支架具有三维连通网状结构,细胞在支架上粘附,生长良好。结论该复合多孔支架具有良好的干细胞亲和性和生物相容性。Objective To probe the preparation and stem cells biocompatibility of the porous HAP/β-TCP/CS scaffold. Method The hydrochemical method was applied to obtain the hydroxyapatite micro-powder. The foam dipping method and the foaming method were adopted to fabricate the porous hydroxyapatite and Beta - tricalcium phosphate scaffold. The ceramic scaffold then was modified with chitin solution to prepare the organic - inorganic composite scaffold. Then the biocompatibility of the composite scaffold was evaluated in vitro via the growth of the human mesenchymal stem cells(MSCs) onto the scaffolds. Results chitin can increase the compressive strength of the HAP/β-TCP scaffold. The scaffold has a three-dimensional and connective network configuration. The adhesion and growth of cells on the scaffold are good. Conclusion The scaffold has good stem cells biocompatibility.

关 键 词：羟基磷灰石粉体 磷酸三钙 甲壳素 复合多孔支架材料 生物相容性 相容性研究 骨髓间质干细胞 复合支架 有机泡沫浸渍 细胞相容性 细胞亲和性 网状结构 体外培养 实验评价 抗压强度 工艺制备 复合成型 支架上 粘附 三维 

分 类 号：R6[医药卫生—外科学]