组织工程肌腱种子细胞的比较研究  

A Comparison Of Dermal Fibroblasts, Bone Marrow-Derived Stem Cells (BMSCs) And Tenocytes As Seed Cells For Tendon Reconstruction

作　　者：李鹏程[1] 程代薇[2] 徐福[1] 魏娴[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科 [2]贵州遵义医学院,贵州省遵义市563003

出　　处：《组织工程与重建外科杂志》2006年第3期134-136,共3页Journal of Tissue Engineering and Reconstructive Surgery

摘　　要：目的探讨猪肌腱细胞、真皮成纤维细胞、骨髓间充质干细胞中何种细胞最适宜作为体外组织工程化肌腱构建的种子细胞。方法收集猪肌腱细胞、真皮成纤维细胞及骨髓间充质干细胞,以50×106个细胞密度均匀接种于圆柱状聚羟基乙酸(Polyglycolic acids,PGA)上,按细胞种类分为三组,每组n=3,体外培养,并于1、2、6周取材,进行组织学检测、免疫组化检测、胶原定量测定和大体观察。结果细胞-PGA复合物体外培养时有细胞外基质产生,六周时肌腱细胞组产生胶原量最多,明显优于真皮成纤维细胞和骨髓间充质干细胞(p<0．01)。免疫组化显示形成的主要为Ⅰ型胶原。结论体外构建组织工程肌腱时肌腱细胞合成胶原能力最强．在现有条件下是体外构建组织工程肌腱的最佳种子细胞。Objective To determine the optimal cell source for tissue engineering of tendons in vitro. Methods Tenocytes, skin fibroblasts, BMSCs, and chondrocytes of passage 2 were seeded at a density of 50xl06cells/ml to PGA scaffold. Specimens were divided into 3 groups according to cell type, and cultured in vitro. Comparison was made between three types of cells using histology, immunohistochemistry, collagen quantification, gross view. Results All cell types tested attached to the PGA material, proliferated, and synthesized extracellular matrices. At week 6, the scaffolds seeded with tenocytes showed the highest collagen production (p<0.01). Scaffolds seeded with skin fibroblasts showed the lowest collagen production. Accordingly, tenocytes appear to be the most suitable cells for further study and for tendon engineering. Conclusion The scaffolds seeded with pig tenocytes show the highest collagen production in vitro. Tenocytes appear to be the most suitable cell source for further study of tendon engineering.

关 键 词：组织工程化 肌腱种子细胞 比较研究 DERMAL Fibroblasts 真皮成纤维细胞 肌腱细胞 骨髓间充质干细胞 体外培养 体外构建 免疫组化检测 胶原量 骨髓问充质干细胞 细胞外基质 聚羟基乙酸 细胞种类 细胞密度 细胞合成 肌腱构建 定量测定 组织学 

分 类 号：R6[医药卫生—外科学]