串联重复信号放大系统检测HBV DNA与其他方法的比较研究  

The comparison and evaluation of the TRSE-Liquid hybridization assay with other three assays for hepatitis B virus

作　　者：顾琳[1] 彭晓谋[1] 张宇峰[1] 陈雪娟[1] 李建国[1] 黄仰甦[1] 高志良[1] 

机构地区：[1]广州中山大学附属第三医院传染病科,510630

出　　处：《中华检验医学杂志》2005年第8期817-820,共4页Chinese Journal of Laboratory Medicine

基　　金：广东省重点攻关项目(99M04801G);中山大学"211工程"项目(98014)

摘　　要：目的探讨TRSE液相杂交法检测HBVDNA的临床应用价值。方法先用TRSE法、斑点杂交法、定性PCR法及荧光定量TaqmanPCR法分别检测倍比稀释的标准HBVDNA质粒,比较其敏感性;再用355份血清标本对各个方法的敏感性、特异度、结果的符合率等做进一步的比较分析。结果荧光定量PCR法的敏感性最高,达到1.33×104copy/ml;普通PCR法的敏感性为5.15×104copy/ml;TRSE法的敏感性为8.33×104copy/ml较斑点杂交法的6.25×105copy/ml高。在HBsAg+HBeAg阳性组,TRES液相杂交法的阳性率与普通PCR法无差别;在HBsAg(+)/HBeAg(-)组,TRSE液相杂交法只与荧光定量TaqmanPCR法有差别。结论TRSE杂交法能够区分在HBeAg阳性和阴性组HBVDNA复制的差别;它具有敏感性、特异度高,实用性强等特点,是一种较好的HBVDNA检测方法。Objective To evaluate the applications of the tandem repeat signal enhanced （TRSE）-liquid hybridization assay for hepatitis B virus in clinical use. Methods A series diluent positive standard were tested by the TRSE- liquid hybridization assay, dot hybridization assay , qualification PCR and Taqman assay. Then all the 355 sera were tested by the 4 assays. Results The Taqman assay has the best sensitivity of 1.33 × 10^4copy/ml of all the 4 assays. The sensitivity of qualification PCR is 5.15 × 10^4copy/ml, just come behind it. The sensitivity of TRSE - liquid phase hybridization assay is 8. 33 × 10^4copy/ml, better than the dot hybridization （6. 25 × 10^5copy/ml）. In the group of HBsAg ＋ HBeAg positive, the positive rate of the TRSE - liquid phase hybridization assay had no different with the rate of qualification PCR assay. In the group of HBsAg （ ＋ ）/HBeAg （-）, the TRSA assay was only different with the Taqman assay. Conclusions TRSE-liquid hybridization assay for HBV DNA could identify the difference in replication of HBV DNA in the groups of HBeAg positive and negative. It was of good sensitivity, specificity,and utility, and could meet the demands of the variety of medical services.

关 键 词：串联重复序列 核酸杂交 肝炎病毒 乙型 聚合酶链反应 DNA质粒 系统检测 HBV 荧光定量PCR法 信号放大 HBEAG阳性 

分 类 号：R446.5[医药卫生—诊断学]